About: Haplotyping barley bmy1 using the SNaPshot assay.

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The allelic status at bmy1 encodes the enzyme beta-amylase 1 in the barley grain and cultivar's malting quality. Changes in the malting process have been responsible for the need to improve the thermostability of this enzyme. We have compared a published bmy1 haplotyping assay based on TDI-FRET with a SNaPshot protocol by jointly analysing a set of 21 cultivars of known haplotype. The SNaPshot assay was therefore used to haplotype the Czech malting barley core collection with respect to bmy1. The old Czech cultivar Kasticky was the only entry identified as carrying the high thermostability haplotype, with the remainder carrying either the intermediate or the low thermostability haplotypes. Older materials were the most variable in terms of bmy1 haplotype, but the majority carried the intermediate type. Most of the descendants of cv. Diamant carried the low thermostability haplotype. The most recently released cultivars recommended for the brewing of Czech beer tend to carry the intermediate allele. The allelic status at bmy1 encodes the enzyme beta-amylase 1 in the barley grain and cultivar's malting quality. Changes in the malting process have been responsible for the need to improve the thermostability of this enzyme. We have compared a published bmy1 haplotyping assay based on TDI-FRET with a SNaPshot protocol by jointly analysing a set of 21 cultivars of known haplotype. The SNaPshot assay was therefore used to haplotype the Czech malting barley core collection with respect to bmy1. The old Czech cultivar Kasticky was the only entry identified as carrying the high thermostability haplotype, with the remainder carrying either the intermediate or the low thermostability haplotypes. Older materials were the most variable in terms of bmy1 haplotype, but the majority carried the intermediate type. Most of the descendants of cv. Diamant carried the low thermostability haplotype. The most recently released cultivars recommended for the brewing of Czech beer tend to carry the intermediate allele. (en)
Title	Haplotyping barley bmy1 using the SNaPshot assay. Haplotyping barley bmy1 using the SNaPshot assay. (en)
skos:prefLabel	Haplotyping barley bmy1 using the SNaPshot assay. Haplotyping barley bmy1 using the SNaPshot assay. (en)
skos:notation	RIV/25328859:_____/10:#0000530!RIV11-GA0-25328859
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA521/07/1028)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Vaculová, Kateřina Milotová, Jarmila
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Agrotest fyto, s.r.o.
http://linked.open...dnocenehoVysledku	261191
http://linked.open...ai/riv/idVysledku	RIV/25328859:_____/10:#0000530
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	barley; beta-amylase; single nucleotide polymorphism (en)
http://linked.open.../riv/klicoveSlovo	single nucleotide polymorphism barley beta-amylase
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[471089E3804B]
http://linked.open...i/riv/nazevZdroje	Biologia
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Expression analysis of barley beta-amylase gene in relation to sequence differences and grain quality
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	65
http://linked.open...iv/tvurceVysledku	Ovesná, Jaroslava Vaculová, Kateřina Milotová, Jarmila Strymplová Šťastná, Kamila
http://linked.open...ain/vavai/riv/wos	000273308400010
issn	0006-3088
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Haplotyping barley bmy1 using the SNaPshot assay. Haplotyping barley bmy1 using the SNaPshot assay. Haplotyping barley bmy1 using the SNaPshot assay.

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