About: Quantification of Pyrenophora teres in infected barley leaves using real-time PCR

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Quantification of Pyrenophora teres in infected barley leaves using real-time PCR Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Net blotch is a barley foliar disease caused by two forms of Pyrenophora teres: Pyrenophora teres f. teres (PTT) and Pyrenophora teres f. maculata (PTM). To monitor and quantify their occurence during the growing season, diagnostic system based on real-time PCR was developed. TaqMan MGB primers and probes were designed that showed high specificity for each of the two forms of P. teres. As a host plant internal standard, TaqMan MGB primers and probe based on RacB gene sequence were designed. The method was optimised on pure fungal DNA and on plasmid standard dilutions. Quantification was accomplished by comparing Ct values of unknown samples with those obtained from plasmids standard dilutions. The assay detects down to five gene copies per reaction. It is able to produce reliable quantitative data over a range of six orders of magnitude. The developed assay was used to differentiate and quantify both forms of P. teres in infected barley leaves. Net blotch is a barley foliar disease caused by two forms of Pyrenophora teres: Pyrenophora teres f. teres (PTT) and Pyrenophora teres f. maculata (PTM). To monitor and quantify their occurence during the growing season, diagnostic system based on real-time PCR was developed. TaqMan MGB primers and probes were designed that showed high specificity for each of the two forms of P. teres. As a host plant internal standard, TaqMan MGB primers and probe based on RacB gene sequence were designed. The method was optimised on pure fungal DNA and on plasmid standard dilutions. Quantification was accomplished by comparing Ct values of unknown samples with those obtained from plasmids standard dilutions. The assay detects down to five gene copies per reaction. It is able to produce reliable quantitative data over a range of six orders of magnitude. The developed assay was used to differentiate and quantify both forms of P. teres in infected barley leaves. (en) Kvantifikace Pyrenophora teres v listech infikovaného ječmene s využitím RT-PCR. (cs)
Title	Quantification of Pyrenophora teres in infected barley leaves using real-time PCR Quantification of Pyrenophora teres in infected barley leaves using real-time PCR (en) Kvantifikace Pyrenophora teres v listech infikovaného ječmene s využitím RT-PCR (cs)
skos:prefLabel	Quantification of Pyrenophora teres in infected barley leaves using real-time PCR Quantification of Pyrenophora teres in infected barley leaves using real-time PCR (en) Kvantifikace Pyrenophora teres v listech infikovaného ječmene s využitím RT-PCR (cs)
skos:notation	RIV/25328859:_____/06:#0000299!RIV08-MZE-25328859
http://linked.open.../vavai/riv/strany	446-455
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QC1361)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Minaříková, Věra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Agrotest fyto, s.r.o.
http://linked.open...dnocenehoVysledku	496185
http://linked.open...ai/riv/idVysledku	RIV/25328859:_____/06:#0000299
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Pyrenophora teres; barley leaves; real-time PCR (en)
http://linked.open.../riv/klicoveSlovo	Pyrenophora teres real-time PCR barley leaves
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[A4B0037642EF]
http://linked.open...i/riv/nazevZdroje	Journal of Microbiological Methods
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Developent of a technique for the quantification of fungal pathogens based on Real-Time PCR approach.
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	67
http://linked.open...iv/tvurceVysledku	Kučera, L. Minaříková, Věra Ovesná, J. Leišová, L.
issn	0167-7012
number of pages	10 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Quantification of Pyrenophora teres in infected barley leaves using real-time PCR Quantification of Pyrenophora teres in infected barley leaves using real-time PCR

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 112 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software