About: AFLP-based PCR markers that differentiate spot and net forms of Pyrenophora teres

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About: AFLP-based PCR markers that differentiate spot and net forms of Pyrenophora teres Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Specific polymerase chain reaction (PCR) primers were developed from amplified fragment length polymorphism (AFLP) fragments of Pyrenophora teres, the causal agent of net blotch on barley leaves. The primers were designed specifically to amplify DNA from P. teres f. teres (net form) and allow its differentiation from P. teres f. maculata (spot form), which is morphologically very similar to P. teres f. teres in culture. The PCR amplification was carried out successfully from DNA extracted from fungal mycelium. The PCR assay was validated with 60 samples of P. teres species. The amplification with four designed PCR primer pairs provided P. teres form-specific products. No cross-reaction was observed with DNA of several other species, such as P. tritici-repentis, P. graminea and Helminthosporium sativum. Specific polymerase chain reaction (PCR) primers were developed from amplified fragment length polymorphism (AFLP) fragments of Pyrenophora teres, the causal agent of net blotch on barley leaves. The primers were designed specifically to amplify DNA from P. teres f. teres (net form) and allow its differentiation from P. teres f. maculata (spot form), which is morphologically very similar to P. teres f. teres in culture. The PCR amplification was carried out successfully from DNA extracted from fungal mycelium. The PCR assay was validated with 60 samples of P. teres species. The amplification with four designed PCR primer pairs provided P. teres form-specific products. No cross-reaction was observed with DNA of several other species, such as P. tritici-repentis, P. graminea and Helminthosporium sativum. (en) Byly vytvořeny primery PCR z AFLP fragmentů Pyrenophora teres, patogena hnědé skvrnitosti ječmene. Primery byly navrženy tak, aby amplifikovaly DNA z P. teres f. teres (síťová forma) a umožnily její odlišení od P. teres f. maculata (skvrnitá forma), která má kulturu morfologicky podobnou P. teres f. teres. Z DNA izolované z mycelia houby byla úspěšně provedena PCR amplifikace. Metoda PCR byla ověřena pomocí 60 vzorků druhu P. teres. Amplifikace čtyř párů PCR primerů vytvořila specifické produkty pro P. teres. Nebyla pozorována žádná křížová reakce s DNA několika jiných druhů, jako např. P. tritici-repentis, P. graminea a Helminthosporium sativum. (cs)
Title	AFLP-based PCR markers that differentiate spot and net forms of Pyrenophora teres Markery PCR založené na AFLP, které rozlišují skvrnitou a síťovou formu Pyrenophora teres (cs) AFLP-based PCR markers that differentiate spot and net forms of Pyrenophora teres (en)
skos:prefLabel	AFLP-based PCR markers that differentiate spot and net forms of Pyrenophora teres Markery PCR založené na AFLP, které rozlišují skvrnitou a síťovou formu Pyrenophora teres (cs) AFLP-based PCR markers that differentiate spot and net forms of Pyrenophora teres (en)
skos:notation	RIV/25328859:_____/05:#0000035!RIV06-MSM-25328859
http://linked.open.../vavai/riv/strany	66-73
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QC1361), Z(MSM2532885901)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Minaříková, Věra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Agrotest fyto, s.r.o.
http://linked.open...dnocenehoVysledku	511521
http://linked.open...ai/riv/idVysledku	RIV/25328859:_____/05:#0000035
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PCR markers; Pyrenophora teres (en)
http://linked.open.../riv/klicoveSlovo	Pyrenophora teres PCR markers
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[EA960D224CE5]
http://linked.open...i/riv/nazevZdroje	Plant Pathology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Developent of a technique for the quantification of fungal pathogens based on Real-Time PCR approach.
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	54
http://linked.open...iv/tvurceVysledku	Kučera, Ladislav Ovesná, Jaroslava Minaříková, Věra Leišová, Leona
http://linked.open...n/vavai/riv/zamer	Optimisation of sustainability factors of crop production based on the development of genetic and breeding, diagnostic and decision-making methods
issn	0032-0862
number of pages	8 (xsd:int)
is http://linked.open...avai/riv/vysledek of	AFLP-based PCR markers that differentiate spot and net forms of Pyrenophora teres

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