About: Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato.

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About: Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cílem této práce byla izolace a purifikace hlavního imunodominantního proteinu, Outer surface proteinu C (OspC) ze skupiny Borrelia burgdorferi, původce Lymeského onemocnění. Cílem bylo získat protein v množství a čistotě vhodné pro imunizaci experimentálních zvířat. Pro optimalizaci výnosu byla použita iontová afinitní chromatografie za různých podmínek. Nejlepší účinnost byla dosažena za použití HiTrap Chelating Column. (cs) The aim of this work was isolation and purification of the major immunodominant protein, Outer surface protein C (OspC) of three members of the species group Borrelia burgdorferi, the causative agent of Lyme disease. The aim was to obtain this protein in a quantity and purity sufficient for immunization of experimental animals. For optimalization of protein purification´s yield was used immobilized metal ion affinity chromatography under different conditions. The greatest efficiency was achieved by using of HiTrap Chelating Column under native conditions. The aim of this work was isolation and purification of the major immunodominant protein, Outer surface protein C (OspC) of three members of the species group Borrelia burgdorferi, the causative agent of Lyme disease. The aim was to obtain this protein in a quantity and purity sufficient for immunization of experimental animals. For optimalization of protein purification´s yield was used immobilized metal ion affinity chromatography under different conditions. The greatest efficiency was achieved by using of HiTrap Chelating Column under native conditions. (en)
Title	Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato. Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato. (en) Izolace a purifikace rekombinantního outer surface proteinu C (rOspC) Borrelia burgdorferi sensu lato. (cs)
skos:prefLabel	Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato. Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato. (en) Izolace a purifikace rekombinantního outer surface proteinu C (rOspC) Borrelia burgdorferi sensu lato. (cs)
skos:notation	RIV/25304046:_____/05:#0000008!RIV06-MPO-25304046
http://linked.open.../vavai/riv/strany	261-264
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(FD-K3/100)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Vrzal, Vladimír Tuháčková, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Bioveta, a.s.
http://linked.open...dnocenehoVysledku	525744
http://linked.open...ai/riv/idVysledku	RIV/25304046:_____/05:#0000008
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	OspC; Borrelia burgdorferi; recombinant protein; immobilized metal ion affinity chromatography (en)
http://linked.open.../riv/klicoveSlovo	recombinant protein OspC Borrelia burgdorferi immobilized metal ion affinity chromatography
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[E4C395418455]
http://linked.open...i/riv/nazevZdroje	Biomedical Papers
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	*Biotechnological production of antigens of Borrelia burgdorferi sensu lato for preparation of prototype of vaccine against Lyme borreliosis.
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	149
http://linked.open...iv/tvurceVysledku	Raška, Milan Weigl, Evžen Křupka, Michal Vrzal, Vladimír Běláková, Jana Tuháčková, Jana Šebestová, Martina
issn	1213-8118
number of pages	4 (xsd:int)

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