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rdf:type
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Description
| - Shoot tips of apple cv. Rubin were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg /L BAP and 0.1 mg /L IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg/ L BAP, which produced 4.2 +/- 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. Rubin were cultivated for 4 weeks at low temperature (4 degrees C) and short photoperiod (8/16-h - light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. The lethal temperature (LT) was 13.0 degrees C for cold hardened and -6.5 degrees C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of cv. Rubin was 78% after thawing in 40 degrees C water.
- Shoot tips of apple cv. Rubin were successfully established in vitro. Five proliferation MS (Murashige and Skoog) media containing different concentrations of BAP (0.25, 0.5, 1, 2 mg L-1) or a combination of 1 mg /L BAP and 0.1 mg /L IBA were tested. The highest proliferation rate was obtained on MS medium with 1 mg/ L BAP, which produced 4.2 +/- 0.3 shoots/explant after one month of cultivation. In vitro plants of cv. Rubin were cultivated for 4 weeks at low temperature (4 degrees C) and short photoperiod (8/16-h - light/dark). The assessment of frost resistance of cold hardened and non-hardened in vitro apple plants was done by freezing tests. The lethal temperature (LT) was 13.0 degrees C for cold hardened and -6.5 degrees C for non-hardened in vitro plants. Cold hardened in vitro cultures were used for subsequent cryopreservation. Cryopreservation was made by encapsulation-dehydration vitrification method. The survival and regeneration of cv. Rubin was 78% after thawing in 40 degrees C water. (en)
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Title
| - Proliferation and cold hardening of in vitro grown apple shoot tips
- Proliferation and cold hardening of in vitro grown apple shoot tips (en)
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skos:prefLabel
| - Proliferation and cold hardening of in vitro grown apple shoot tips
- Proliferation and cold hardening of in vitro grown apple shoot tips (en)
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skos:notation
| - RIV/25271121:_____/06:0R000945!RIV10-MZE-25271121
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/25271121:_____/06:0R000945
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - micropropagation; germplasm; encapsulation; cryopreservation; freezing (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Zámečník, Jiří
- Paprštein, František
- Sedlák, Jiří
- Bilavčík, Alois
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http://linked.open...vavai/riv/typAkce
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http://linked.open...ain/vavai/riv/wos
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http://linked.open.../riv/zahajeniAkce
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number of pages
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http://purl.org/ne...btex#hasPublisher
| - International Society for Horticultural Science
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https://schema.org/isbn
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is http://linked.open...avai/riv/vysledek
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