About: Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs

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About: Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1 Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.brewingscience.de/abstracts.php3
Description	Complex lupulin gland-specific cDNA library from Osvald's clone 72 hop was constructed to dissect regulation of biosynthetic pathway(s) leading to the accumulation of hop bitter acids and prenylflavonoids with promising health-beneficial activities. From this cDNA library we isolated first hop-specific allelic isoforms of transcription factors (TF) bHLH (HlbHLH2, GeneBank AC:FR751553) and WD40 (HlWD40_1, AC:NM_122360) which are involved in combinatorial control of light-responsive and tissue-specific activation of phenylpropanoid pathway. HlbHLH2 and HlWD40_1 are quite lupulin gland-specific and, according to our transient expression experiments, they form specific complexes with another novel hop TF HlMyb2 (AC:FN646081) and previously isolated lupulin gland-specific HlMyb3 (AC:AM501509). The complex formation leads to strong activation of chalconsynthase gene (chs_H1) promoter. The interplay and regulation of expression of these crucial TF complexes could co-determine the rate of accumulation of valuable metabolites of lupulin. Complex lupulin gland-specific cDNA library from Osvald's clone 72 hop was constructed to dissect regulation of biosynthetic pathway(s) leading to the accumulation of hop bitter acids and prenylflavonoids with promising health-beneficial activities. From this cDNA library we isolated first hop-specific allelic isoforms of transcription factors (TF) bHLH (HlbHLH2, GeneBank AC:FR751553) and WD40 (HlWD40_1, AC:NM_122360) which are involved in combinatorial control of light-responsive and tissue-specific activation of phenylpropanoid pathway. HlbHLH2 and HlWD40_1 are quite lupulin gland-specific and, according to our transient expression experiments, they form specific complexes with another novel hop TF HlMyb2 (AC:FN646081) and previously isolated lupulin gland-specific HlMyb3 (AC:AM501509). The complex formation leads to strong activation of chalconsynthase gene (chs_H1) promoter. The interplay and regulation of expression of these crucial TF complexes could co-determine the rate of accumulation of valuable metabolites of lupulin. (en)
Title	Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1 Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1 (en)
skos:prefLabel	Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1 Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1 (en)
skos:notation	RIV/14864347:_____/11:#0000346!RIV12-MZE-14864347
http://linked.open...avai/predkladatel	Chmelařský institut s.r.o.
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA521/08/0740), P(QH81052)
http://linked.open...iv/cisloPeriodika	11-12
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Patzak, Josef
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Chmelařský institut s.r.o.
http://linked.open...dnocenehoVysledku	200516
http://linked.open...ai/riv/idVysledku	RIV/14864347:_____/11:#0000346
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Humulus lupulus L.; lupulin metabolome; transcription factors and protein complexes; transient expression assay in N. benthamiana (en)
http://linked.open.../riv/klicoveSlovo	Humulus lupulus L. lupulin metabolome transcription factors and protein complexes transient expression assay in N. benthamiana
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[1E59C20B80D1]
http://linked.open...i/riv/nazevZdroje	Brewing Science
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular analysis of transcription factors of hop (H. lupulus L.) in relation to lupulin biosynthesis Development of molecular-genetic markers for modern breeding and gene engineering of hop (Humulus lupulus) based on the system of genomic and expression libraries.
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	64
http://linked.open...iv/tvurceVysledku	Matoušek, Jaroslav Patzak, Josef Duraisamy, G. S. Füssy, Zoltán Kocábek, Tomáš Orctová, Lidmila Stehlík, Jan
issn	0723-1520
number of pages	5 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1 Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1

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