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  • A new trend in molecular methods is to develop new molecular markers in specific sequences, near or within structural genes. Recently, markers in intron, 5’ and 3’UTR were identified in several hop genes. Increasing sequence information of hop genes in Genebank provides additional opportunities to develop new gene specific molecular markers. In this study, we report new efficient and reliable expressed sequence tag microsatellite repeat (EST-SSR) molecular markers. We used forty primer pairs for twenty-nine gene specific sequences. We evaluated them for identification and clustering in eight hops cultivars. Gene-specific based clustering was compared to clustering based on SSR, AFLP markers and on a combination of molecular markers. These molecular markers are ideal for DNA fingerprinting, for control of authenticity and purity of varieties, for evaluation of genetic diversity in genetic resources, etc.
  • A new trend in molecular methods is to develop new molecular markers in specific sequences, near or within structural genes. Recently, markers in intron, 5’ and 3’UTR were identified in several hop genes. Increasing sequence information of hop genes in Genebank provides additional opportunities to develop new gene specific molecular markers. In this study, we report new efficient and reliable expressed sequence tag microsatellite repeat (EST-SSR) molecular markers. We used forty primer pairs for twenty-nine gene specific sequences. We evaluated them for identification and clustering in eight hops cultivars. Gene-specific based clustering was compared to clustering based on SSR, AFLP markers and on a combination of molecular markers. These molecular markers are ideal for DNA fingerprinting, for control of authenticity and purity of varieties, for evaluation of genetic diversity in genetic resources, etc. (en)
Title
  • Gene specific molecular markers for hop (Humulus lupulus L.)
  • Gene specific molecular markers for hop (Humulus lupulus L.) (en)
skos:prefLabel
  • Gene specific molecular markers for hop (Humulus lupulus L.)
  • Gene specific molecular markers for hop (Humulus lupulus L.) (en)
skos:notation
  • RIV/14864347:_____/09:#0000229!RIV10-GA0-14864347
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA521/08/0740), P(QH81052)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 316055
http://linked.open...ai/riv/idVysledku
  • RIV/14864347:_____/09:#0000229
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • expressed sequence tag (EST); single nucleotide polymorphism (SNP); microsatellite repeat; simple sequence repeat (SSR); insertion/deletion (indel); cluster analysis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [B4D2D1CDC864]
http://linked.open...i/riv/mistoVydani
  • Leuven, Belgie
http://linked.open...vEdiceCisloSvazku
  • Acta Horticulturae 848
http://linked.open...i/riv/nazevZdroje
  • Proceedings of the Second International Humulus Symposium
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...v/pocetStranKnihy
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Matoušek, Jaroslav
  • Patzak, Josef
number of pages
http://purl.org/ne...btex#hasPublisher
  • International Society for Horticultural Science
https://schema.org/isbn
  • 9789066057227
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