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  • Solid phase magnetic driven separation techniques in combination with molecular diagnostic methods (polymerase chain reaction, PCR) have become popular to speed up and facilitate the nucleic acids separation and purification procedures. Magnetic particles are mostly based on magnetic iron oxide (magnetic core) covered by polymer surfaces suitable for modification by different functional groups (e.g. –COOH, -NH2). Free or incomplete encapsulated cores and some components applied in the preparation of magnetic particles can interfere with polymerase chain reaction [1, 2]. Therefore perfectly encapsulation of magnetic cores and removing all potential PCR inhibiting components are key factors for the application of magnetic particles in molecular diagnostics. For this reasons, polymerase chain reaction in quantitative real time (qPCR) and the software supplied with the qPCR, were used for the estimation of the inhibitory effect of different types of magnetic microparticles. The set of magnetic non-por
  • Solid phase magnetic driven separation techniques in combination with molecular diagnostic methods (polymerase chain reaction, PCR) have become popular to speed up and facilitate the nucleic acids separation and purification procedures. Magnetic particles are mostly based on magnetic iron oxide (magnetic core) covered by polymer surfaces suitable for modification by different functional groups (e.g. –COOH, -NH2). Free or incomplete encapsulated cores and some components applied in the preparation of magnetic particles can interfere with polymerase chain reaction [1, 2]. Therefore perfectly encapsulation of magnetic cores and removing all potential PCR inhibiting components are key factors for the application of magnetic particles in molecular diagnostics. For this reasons, polymerase chain reaction in quantitative real time (qPCR) and the software supplied with the qPCR, were used for the estimation of the inhibitory effect of different types of magnetic microparticles. The set of magnetic non-por (en)
Title
  • Study on the encapsulation of magnetic cores using real time polymerase chain reaction
  • Study on the encapsulation of magnetic cores using real time polymerase chain reaction (en)
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  • Study on the encapsulation of magnetic cores using real time polymerase chain reaction
  • Study on the encapsulation of magnetic cores using real time polymerase chain reaction (en)
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  • RIV/00216305:26310/14:PU112201!RIV15-GA0-26310___
http://linked.open...avai/riv/aktivita
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  • P(GP13-32840P)
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  • 48387
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  • RIV/00216305:26310/14:PU112201
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  • magnetic particles, PCR magnetic cores encapsulation (en)
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  • [8324B0D63228]
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  • Horák, Daniel
  • Rittich, Bohuslav
  • Trachtová, Štěpánka
  • Španová, Alena
http://localhost/t...ganizacniJednotka
  • 26310
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