About: USE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR DETERMINATION OF SACCHARIDES AFTER THE ENZYMATIC HYDROLYSIS OF WASTE BREAD

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf
Description	The main topic of our study was the effect of pH and temperature on the efficiency of enzymatic hydrolysis. For study of enzymatic hydrolysis were used spectrophotometric and HPLC methods. Optimal value of pH and temperature of liquefaction was determined spectrophotometrically by Somogyi-Nelson method. Final yields of glucose from hydrolysis and efficiency of saccharification were determined by HPLC method. As substrate for enzymatic hydrolysis was used waste bread. It was pre-treated by grinding into small particles and then it was made to form 15% w/v suspension. For the hydrolysis have been used two enzymes. For liquefaction of the substrate we used ? amylase, and for saccharification we used glucoamylase. It was found that optimal conditions for ? amylase were pH 6 and temperature 80 °C. Under these conditions the yield of reducing sugars was 67.08 gL-1 (calculated to maltose). Optimal conditions for glucoamylase were pH 4.2 and temperature 60 °C. Obtained yield of glucose was 70.28 gL The main topic of our study was the effect of pH and temperature on the efficiency of enzymatic hydrolysis. For study of enzymatic hydrolysis were used spectrophotometric and HPLC methods. Optimal value of pH and temperature of liquefaction was determined spectrophotometrically by Somogyi-Nelson method. Final yields of glucose from hydrolysis and efficiency of saccharification were determined by HPLC method. As substrate for enzymatic hydrolysis was used waste bread. It was pre-treated by grinding into small particles and then it was made to form 15% w/v suspension. For the hydrolysis have been used two enzymes. For liquefaction of the substrate we used ? amylase, and for saccharification we used glucoamylase. It was found that optimal conditions for ? amylase were pH 6 and temperature 80 °C. Under these conditions the yield of reducing sugars was 67.08 gL-1 (calculated to maltose). Optimal conditions for glucoamylase were pH 4.2 and temperature 60 °C. Obtained yield of glucose was 70.28 gL (en)
Title	USE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR DETERMINATION OF SACCHARIDES AFTER THE ENZYMATIC HYDROLYSIS OF WASTE BREAD USE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR DETERMINATION OF SACCHARIDES AFTER THE ENZYMATIC HYDROLYSIS OF WASTE BREAD (en)
skos:prefLabel	USE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR DETERMINATION OF SACCHARIDES AFTER THE ENZYMATIC HYDROLYSIS OF WASTE BREAD USE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR DETERMINATION OF SACCHARIDES AFTER THE ENZYMATIC HYDROLYSIS OF WASTE BREAD (en)
skos:notation	RIV/00216305:26310/14:PU112104!RIV15-MSM-26310___
http://linked.open...avai/riv/aktivita	S
http://linked.open...avai/riv/aktivity	S
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Šupinová, Petra Babák, Libor Hudečková, Helena
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta chemická
http://linked.open...dnocenehoVysledku	52228
http://linked.open...ai/riv/idVysledku	RIV/00216305:26310/14:PU112104
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	waste bread, enzymatic hydrolysis, amylases, alpha-amylase, glucoamylase, pH, temperature (en)
http://linked.open.../riv/klicoveSlovo	pH alpha-amylase temperature enzymatic hydrolysis amylases glucoamylase waste bread
http://linked.open...ontrolniKodProRIV	[D02952C36642]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...i/riv/nazevZdroje	CECE 2014 11th International Interdisciplinary Meeting on Bioanalysis
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Babák, Libor Šupinová, Petra Hudečková, Helena
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2014-10-20 (xsd:date)
number of pages	475 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Neuveden
https://schema.org/isbn	978-80-904959-2-0
http://localhost/t...ganizacniJednotka	26310

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