About: The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR     Goto   Sponge   NotDistinct   Permalink

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  • Isolation of DNA is an important step in the molecular diagnostics of microorganisms and viruses, medical diagnosis, etc. Different magnetic nano- and microparticles have been used as a solid support for DNA isolation. Magnetic nano- and microparticles usually consist of a number of components: supermagnetic core, protective coating and the surface functionality. Iron oxides and some substances used to stabilize magnetic cores reduce biocompatibility of the proposed carriers, therefore perfectly cover core prerequisite for their application in molecular diagnostics. Magnetic cores are covered with a variety of functionalized low-or high-molecular compounds (polymer layer). The work was focused on the estimation of influence of microparticles with magnetite core on the amplification of DNA. A set of 8 types of hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) – P(HEMA-co-GMA) microparticles, 6 types of poly(glycidyl methacrylate) (PGMA) microparticles and 3 types of
  • Isolation of DNA is an important step in the molecular diagnostics of microorganisms and viruses, medical diagnosis, etc. Different magnetic nano- and microparticles have been used as a solid support for DNA isolation. Magnetic nano- and microparticles usually consist of a number of components: supermagnetic core, protective coating and the surface functionality. Iron oxides and some substances used to stabilize magnetic cores reduce biocompatibility of the proposed carriers, therefore perfectly cover core prerequisite for their application in molecular diagnostics. Magnetic cores are covered with a variety of functionalized low-or high-molecular compounds (polymer layer). The work was focused on the estimation of influence of microparticles with magnetite core on the amplification of DNA. A set of 8 types of hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) – P(HEMA-co-GMA) microparticles, 6 types of poly(glycidyl methacrylate) (PGMA) microparticles and 3 types of (en)
Title
  • The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR
  • The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR (en)
skos:prefLabel
  • The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR
  • The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR (en)
skos:notation
  • RIV/00216305:26310/14:PU109810!RIV15-GA0-26310___
http://linked.open...avai/riv/aktivita
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  • P(GP13-32840P)
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  • 48295
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  • RIV/00216305:26310/14:PU109810
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  • magnetic particles, PCR inhibition (en)
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  • [B1F5629BF229]
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  • Horák, Daniel
  • Rittich, Bohuslav
  • Trachtová, Štěpánka
  • Španová, Alena
http://localhost/t...ganizacniJednotka
  • 26310
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