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  • In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has become a powerful diagnostic tool for the analysis of microorganisms. However, the sensitivity of PCR may be reduced when complex biological samples are studied, since various extracellular PCR inhibitors may be present. The problem can be resolved by means of various DNA isolation and purification methods. Magnetic particles can be successfully used for the isolation of DNA from various samples containing PCR inhibitors. Research has been focused on the development of innovative magnetic nanoparticles.
  • In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has become a powerful diagnostic tool for the analysis of microorganisms. However, the sensitivity of PCR may be reduced when complex biological samples are studied, since various extracellular PCR inhibitors may be present. The problem can be resolved by means of various DNA isolation and purification methods. Magnetic particles can be successfully used for the isolation of DNA from various samples containing PCR inhibitors. Research has been focused on the development of innovative magnetic nanoparticles. (en)
Title
  • Application of real-time PCR for evaluation of magnetic nanoparticles in molecular diagnostics
  • Application of real-time PCR for evaluation of magnetic nanoparticles in molecular diagnostics (en)
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  • Application of real-time PCR for evaluation of magnetic nanoparticles in molecular diagnostics
  • Application of real-time PCR for evaluation of magnetic nanoparticles in molecular diagnostics (en)
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  • RIV/00216305:26310/11:PU93110!RIV12-MSM-26310___
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  • RIV/00216305:26310/11:PU93110
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  • magnetic particles, polymerase chain reaction, probiotic dairy products (en)
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  • Rittich, Bohuslav
  • Trachtová, Štěpánka
  • Španová, Alena
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  • 26310
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