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  • The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. the collection of deletion mutants now covers 99% of the fission yeast open reading frames.
  • The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. the collection of deletion mutants now covers 99% of the fission yeast open reading frames. (en)
Title
  • Finishing the S.pombe genome deletion project. An update.
  • Finishing the S.pombe genome deletion project. An update. (en)
skos:prefLabel
  • Finishing the S.pombe genome deletion project. An update.
  • Finishing the S.pombe genome deletion project. An update. (en)
skos:notation
  • RIV/00216305:26310/10:PU90289!RIV11-MSM-26310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(2B08057)
http://linked.open...iv/cisloPeriodika
  • 12
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 259230
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  • RIV/00216305:26310/10:PU90289
http://linked.open...riv/jazykVysledku
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  • fission yeast, gene deletion, genomics, essential genes, vectors (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [7FF78699BB29]
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  • Cell Cycle
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 9
http://linked.open...iv/tvurceVysledku
  • Márová, Ivana
  • Čarnecká, Martina
issn
  • 1538-4101
number of pages
http://localhost/t...ganizacniJednotka
  • 26310
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