About: The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium

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About: The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study was to select and investigate non-pathogenic bacterial strains of the genus Clostridium collected in the Dairy Research Institute. The strains were isolated from cheeses with late blowing disorders. First, a set of 44 strains was identified into the genus Clostridium using genus-specific PCR (Rekha et al., 2006). PCRs specific to the species Clostridium butyricum (Nakanishi et al., 2005) and Clostridium tyrobutyricum (Herman et al., 1995) were used for species identification. It was found that 7 and 12 strains belonged to the species C. butyricum or C. tyrobutyricum, respectively. Some strains were not identified into species yet. Strains encoding the H2-evolving hydrogenase (hydA) gene were also identified using PCR ( ). Formation of fatty acids (acetic, propionic, and butyric) was studied by gas chromatography in culture supernatants. The aim of this study was to select and investigate non-pathogenic bacterial strains of the genus Clostridium collected in the Dairy Research Institute. The strains were isolated from cheeses with late blowing disorders. First, a set of 44 strains was identified into the genus Clostridium using genus-specific PCR (Rekha et al., 2006). PCRs specific to the species Clostridium butyricum (Nakanishi et al., 2005) and Clostridium tyrobutyricum (Herman et al., 1995) were used for species identification. It was found that 7 and 12 strains belonged to the species C. butyricum or C. tyrobutyricum, respectively. Some strains were not identified into species yet. Strains encoding the H2-evolving hydrogenase (hydA) gene were also identified using PCR ( ). Formation of fatty acids (acetic, propionic, and butyric) was studied by gas chromatography in culture supernatants. (en)
Title	The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium (en)
skos:prefLabel	The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium (en)
skos:notation	RIV/00216305:26310/09:PU87649!RIV11-MSM-26310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(2B08070)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Španová, Alena Rittich, Bohuslav Ürgeová, Barbora
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta chemická
http://linked.open...dnocenehoVysledku	303818
http://linked.open...ai/riv/idVysledku	RIV/00216305:26310/09:PU87649
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Clostridium butyricum, Clostridium tyrobutyricum, hydrogenase (hydA), gas chromatography PCRs, (en)
http://linked.open.../riv/klicoveSlovo	gas chromatography PCRs hydrogenase (hydA) Clostridium tyrobutyricum Clostridium butyricum
http://linked.open...ontrolniKodProRIV	[BAE66BED79E3]
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of new modern biotechnological process for more effective utilization of waste water from dairy industry as material source for hydrogen production.
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Rittich, Bohuslav Španová, Alena Ürgeová, Barbora
http://localhost/t...ganizacniJednotka	26310

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