About: DNA Analysis of Clostridium Isolated from Late-blowing Cheeses.

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About: DNA Analysis of Clostridium Isolated from Late-blowing Cheeses. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Some species of genus Clostridium provoke the late-blowing defect in cheeses which is characterized by high concentration of butyric acid and production of hydrogen and carbon dioxide. Therefore, the presence of butyric acid bacteria and spores in milk is undesirable and needs to be determined. This work is focused on the DNA analysis of a collection of bacteria isolated from late-blowing cheeses. PCR methods for genus and species identification were applied. Using genus-specific PCR, the set of 45 DNA samples was identified as DNA of Clostridium genus. Based on species-specific PCRs, it was found out that some of the samples were mixtures from C. butyricum and C. tyrobutyricum cultures. These samples were purified in order to obtain exact species classification. It was determined that 6 strains belong to the species Clostridium butyricum and 27 strains belong to the species Clostridium tyrobutyricum. Another 12 samples were not classified into tested species. Strains with the hydrogenase (hydA) gene Some species of genus Clostridium provoke the late-blowing defect in cheeses which is characterized by high concentration of butyric acid and production of hydrogen and carbon dioxide. Therefore, the presence of butyric acid bacteria and spores in milk is undesirable and needs to be determined. This work is focused on the DNA analysis of a collection of bacteria isolated from late-blowing cheeses. PCR methods for genus and species identification were applied. Using genus-specific PCR, the set of 45 DNA samples was identified as DNA of Clostridium genus. Based on species-specific PCRs, it was found out that some of the samples were mixtures from C. butyricum and C. tyrobutyricum cultures. These samples were purified in order to obtain exact species classification. It was determined that 6 strains belong to the species Clostridium butyricum and 27 strains belong to the species Clostridium tyrobutyricum. Another 12 samples were not classified into tested species. Strains with the hydrogenase (hydA) gene (en)
Title	DNA Analysis of Clostridium Isolated from Late-blowing Cheeses. DNA Analysis of Clostridium Isolated from Late-blowing Cheeses. (en)
skos:prefLabel	DNA Analysis of Clostridium Isolated from Late-blowing Cheeses. DNA Analysis of Clostridium Isolated from Late-blowing Cheeses. (en)
skos:notation	RIV/00216305:26310/09:PU84184!RIV10-MSM-26310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(2B08070)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Španová, Alena Rittich, Bohuslav Ürgeová, Barbora
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Fakulta chemická
http://linked.open...dnocenehoVysledku	310995
http://linked.open...ai/riv/idVysledku	RIV/00216305:26310/09:PU84184
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PCR, Clostridium butyricum, Clostridium tyrobutyricum, hydA gene (en)
http://linked.open.../riv/klicoveSlovo	PCR hydA gene Clostridium tyrobutyricum Clostridium butyricum
http://linked.open...ontrolniKodProRIV	[D6A3C8F2B0FD]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Fakulta chemická Purkyňova 464/118 61200 Brno
http://linked.open...i/riv/nazevZdroje	Studentská odborná konference chemie a společnost
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of new modern biotechnological process for more effective utilization of waste water from dairy industry as material source for hydrogen production.
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Rittich, Bohuslav Španová, Alena Ürgeová, Barbora
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2009-12-03 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Vysoké učení technické v Brně
https://schema.org/isbn	978-80-214-4008-1
http://localhost/t...ganizacniJednotka	26310
is http://linked.open...avai/riv/vysledek of	DNA Analysis of Clostridium Isolated from Late-blowing Cheeses. DNA Analysis of Clostridium Isolated from Late-blowing Cheeses. DNA Analysis of Clostridium Isolated from Late-blowing Cheeses. DNA Analysis of Clostridium Isolated from Late-blowing Cheeses.

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