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rdf:type
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Description
| - Storing of living cells in liquid nitrogen (LN2) has been a standard for a long time. Disadvantages are well known – LN2 evaporates. There is another possibility to store living cells using a freezer with temperatures of -80. In this work, we focus on the study of HEK293 cells stored in the low temperature freezer and evaluation of cell quality. Our experiments involved 190 individual cells, 87 of them frozen for ten days. Then, cells were thawed and their ability to establish a gigaseal in patch-clamp measurement was tested. Ten-day freezing did not influenced quality of cells comparing to those stored in LN2 but there were small differences comparing to control group of cells.
- Storing of living cells in liquid nitrogen (LN2) has been a standard for a long time. Disadvantages are well known – LN2 evaporates. There is another possibility to store living cells using a freezer with temperatures of -80. In this work, we focus on the study of HEK293 cells stored in the low temperature freezer and evaluation of cell quality. Our experiments involved 190 individual cells, 87 of them frozen for ten days. Then, cells were thawed and their ability to establish a gigaseal in patch-clamp measurement was tested. Ten-day freezing did not influenced quality of cells comparing to those stored in LN2 but there were small differences comparing to control group of cells. (en)
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Title
| - Effect of Deep Freezing To Quality of HEK293 Transfected With CAV 3.1 Membrane Channel
- Effect of Deep Freezing To Quality of HEK293 Transfected With CAV 3.1 Membrane Channel (en)
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skos:prefLabel
| - Effect of Deep Freezing To Quality of HEK293 Transfected With CAV 3.1 Membrane Channel
- Effect of Deep Freezing To Quality of HEK293 Transfected With CAV 3.1 Membrane Channel (en)
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skos:notation
| - RIV/00216305:26220/14:PU108842!RIV15-MSM-26220___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216305:26220/14:PU108842
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - cell freezing, HEK 293, CaV 3.1, membrane channel, patch clamp, transfection (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Hubálek, Jaromír
- Provazník, Ivo
- Svoboda, Ondřej
- Baiazitova, Larisa
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http://localhost/t...ganizacniJednotka
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