About: Effect of Deep Freezing To Quality of HEK293 Transfected With CAV 3.1 Membrane Channel     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

AttributesValues
rdf:type
Description
  • Storing of living cells in liquid nitrogen (LN2) has been a standard for a long time. Disadvantages are well known – LN2 evaporates. There is another possibility to store living cells using a freezer with temperatures of -80. In this work, we focus on the study of HEK293 cells stored in the low temperature freezer and evaluation of cell quality. Our experiments involved 190 individual cells, 87 of them frozen for ten days. Then, cells were thawed and their ability to establish a gigaseal in patch-clamp measurement was tested. Ten-day freezing did not influenced quality of cells comparing to those stored in LN2 but there were small differences comparing to control group of cells.
  • Storing of living cells in liquid nitrogen (LN2) has been a standard for a long time. Disadvantages are well known – LN2 evaporates. There is another possibility to store living cells using a freezer with temperatures of -80. In this work, we focus on the study of HEK293 cells stored in the low temperature freezer and evaluation of cell quality. Our experiments involved 190 individual cells, 87 of them frozen for ten days. Then, cells were thawed and their ability to establish a gigaseal in patch-clamp measurement was tested. Ten-day freezing did not influenced quality of cells comparing to those stored in LN2 but there were small differences comparing to control group of cells. (en)
Title
  • Effect of Deep Freezing To Quality of HEK293 Transfected With CAV 3.1 Membrane Channel
  • Effect of Deep Freezing To Quality of HEK293 Transfected With CAV 3.1 Membrane Channel (en)
skos:prefLabel
  • Effect of Deep Freezing To Quality of HEK293 Transfected With CAV 3.1 Membrane Channel
  • Effect of Deep Freezing To Quality of HEK293 Transfected With CAV 3.1 Membrane Channel (en)
skos:notation
  • RIV/00216305:26220/14:PU108842!RIV15-MSM-26220___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GAP102/11/1068), S
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 13216
http://linked.open...ai/riv/idVysledku
  • RIV/00216305:26220/14:PU108842
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cell freezing, HEK 293, CaV 3.1, membrane channel, patch clamp, transfection (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [3CFE931C868A]
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Hubálek, Jaromír
  • Provazník, Ivo
  • Svoboda, Ondřej
  • Baiazitova, Larisa
http://localhost/t...ganizacniJednotka
  • 26220
Faceted Search & Find service v1.16.116 as of Feb 22 2024


Alternative Linked Data Documents: ODE     Content Formats:   [cxml] [csv]     RDF   [text] [turtle] [ld+json] [rdf+json] [rdf+xml]     ODATA   [atom+xml] [odata+json]     Microdata   [microdata+json] [html]    About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data] Valid XHTML + RDFa
OpenLink Virtuoso version 07.20.3239 as of Feb 22 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 81 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software