About: Parallel-Mode Confocal Microscopy     Goto   Sponge   NotDistinct   Permalink

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  • Conventional confocal microscopes are based on the dual scanning of a specimen by the images of a point source and of a point detector. Hence, their imaging mode is serial, i.e., the confocal image is obtained from the sequence of single-point or multiple-point partial images. Parallel-mode confocal imaging is possible on the basis of broad-source image-plane holography. We adapted this classical-holography technique for real-time reflected-light microscopy. The imaging speed of the parallel-mode conffocal microscope is not limited by any part of the optical system, but only by the image detection and storage systems. Both the image phase and the image amplitude are reconstructed from the interference signal. It was verified both theoretically and experimentally that the main imaging parameters of the microscope are comparable with those of a conventional confocal microscope. The only exception is the imaging speed, which can be much higher.
  • Conventional confocal microscopes are based on the dual scanning of a specimen by the images of a point source and of a point detector. Hence, their imaging mode is serial, i.e., the confocal image is obtained from the sequence of single-point or multiple-point partial images. Parallel-mode confocal imaging is possible on the basis of broad-source image-plane holography. We adapted this classical-holography technique for real-time reflected-light microscopy. The imaging speed of the parallel-mode conffocal microscope is not limited by any part of the optical system, but only by the image detection and storage systems. Both the image phase and the image amplitude are reconstructed from the interference signal. It was verified both theoretically and experimentally that the main imaging parameters of the microscope are comparable with those of a conventional confocal microscope. The only exception is the imaging speed, which can be much higher. (en)
Title
  • Parallel-Mode Confocal Microscopy
  • Parallel-Mode Confocal Microscopy (en)
skos:prefLabel
  • Parallel-Mode Confocal Microscopy
  • Parallel-Mode Confocal Microscopy (en)
skos:notation
  • RIV/00216305:26210/99:PU20998!RIV/2002/GA0/262102/N
http://linked.open.../vavai/riv/strany
  • 1635-1639
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA202/96/1520), P(GA202/98/P285)
http://linked.open...iv/cisloPeriodika
  • 10
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 749098
http://linked.open...ai/riv/idVysledku
  • RIV/00216305:26210/99:PU20998
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Confocal microscopy, Holographic applications, Real-time imaging. (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [8AE71C051569]
http://linked.open...i/riv/nazevZdroje
  • Optical Engineering
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 38
http://linked.open...iv/tvurceVysledku
  • Chmelík, Radim
  • Harna, Zdeněk
issn
  • 0091-3286
number of pages
http://localhost/t...ganizacniJednotka
  • 26210
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