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Description
  • V tomto článku prezentujeme nově vyvinutý transmisní digitální holografický mikroskop. Mikroskop umožňuje použití světla s různou koherencí (jak časovou, tak I prostorovou) současně s mimoosovou holografií. Je popsáno schema mikroskopu, jeho funkce a rekonstrukce objektové vlny. Je demonstrován jev optických řezů (podobný tomu v konfokálním mikroskopu), který je důsledkem použití světla s nízkou kohernecí. Mikroskop byl použit pro stadium dynamiky živých buněk. Různá chování související se změnami buněčného cyklu jsou zde demonstrovány. (cs)
  • In this paper we present a newly developed digital transmission holographic microscope. The microscope enables using arbitrarily low coherent illumination (both spatially and temporally) in conjunction with the off-axis holography. The setup of the microscope, its function and the object wave reconstruction procedure are described. The optical sectioning effect, similar to a confocal microscope, resulting from the use of low spatially coherent light source is demonstrated. The microscope has been tailored for studies of living cell dynamics. Time-lapse phase reconstruction series of live cells activities were carried out. The different behavior related to changes in the cell cycle is demonstrated.
  • In this paper we present a newly developed digital transmission holographic microscope. The microscope enables using arbitrarily low coherent illumination (both spatially and temporally) in conjunction with the off-axis holography. The setup of the microscope, its function and the object wave reconstruction procedure are described. The optical sectioning effect, similar to a confocal microscope, resulting from the use of low spatially coherent light source is demonstrated. The microscope has been tailored for studies of living cell dynamics. Time-lapse phase reconstruction series of live cells activities were carried out. The different behavior related to changes in the cell cycle is demonstrated. (en)
Title
  • Digital holographic microscope with low-spatial and temporal coherence of illumination
  • Digitální holografická mikroskopie s nízkou prostorovou a časovou koherencí osvětlení (cs)
  • Digital holographic microscope with low-spatial and temporal coherence of illumination (en)
skos:prefLabel
  • Digital holographic microscope with low-spatial and temporal coherence of illumination
  • Digitální holografická mikroskopie s nízkou prostorovou a časovou koherencí osvětlení (cs)
  • Digital holographic microscope with low-spatial and temporal coherence of illumination (en)
skos:notation
  • RIV/00216305:26210/08:PU78125!RIV09-GA0-26210___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA202/08/0590)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 363618
http://linked.open...ai/riv/idVysledku
  • RIV/00216305:26210/08:PU78125
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • digital imaging, holography, low coherence, image reconstruction, live cells (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [EF9EBEF78606]
http://linked.open...v/mistoKonaniAkce
  • Strasbourg
http://linked.open...i/riv/mistoVydani
  • Washington 98227-0010 USA
http://linked.open...i/riv/nazevZdroje
  • Optical and Digital Image Processing
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Chmelík, Radim
  • Veselý, Pavel
  • Kolman, Pavel
  • Janečková, Hana
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • SPIE
https://schema.org/isbn
  • 978-0-8194-7198-7
http://localhost/t...ganizacniJednotka
  • 26210
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