About: Phosphatidylcholines with Interfered Masses of Protonated and Sodiated Molecules by Matrix-assisted Laser Desorption/Ionization Mass Spectroscopy     Goto   Sponge   NotDistinct   Permalink

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Description
  • This work presents a computational method for deconvolution of interfered peaks of protonated and sodiated phosphatidylcholines (PCs) to solve the problem of relative quantitation of these PCs. The method is based on the fact that adducts of PCs with proton and sodium ion give unique characteristic peaks in tandem mass spectra. The intensities of these peaks were used to find out contribution of protonated and sodiated PCs to the formation of interfered peak in full-scan mass spectrum and, further, to correlate peak intensities that correspond to PCs in full-scan mass spectrum with PC's concentration. The model was calibrated on synthesized PCs and verified for applicability in the range of Na+ ion concentrations from trace to physiological one.
  • This work presents a computational method for deconvolution of interfered peaks of protonated and sodiated phosphatidylcholines (PCs) to solve the problem of relative quantitation of these PCs. The method is based on the fact that adducts of PCs with proton and sodium ion give unique characteristic peaks in tandem mass spectra. The intensities of these peaks were used to find out contribution of protonated and sodiated PCs to the formation of interfered peak in full-scan mass spectrum and, further, to correlate peak intensities that correspond to PCs in full-scan mass spectrum with PC's concentration. The model was calibrated on synthesized PCs and verified for applicability in the range of Na+ ion concentrations from trace to physiological one. (en)
Title
  • Phosphatidylcholines with Interfered Masses of Protonated and Sodiated Molecules by Matrix-assisted Laser Desorption/Ionization Mass Spectroscopy
  • Phosphatidylcholines with Interfered Masses of Protonated and Sodiated Molecules by Matrix-assisted Laser Desorption/Ionization Mass Spectroscopy (en)
skos:prefLabel
  • Phosphatidylcholines with Interfered Masses of Protonated and Sodiated Molecules by Matrix-assisted Laser Desorption/Ionization Mass Spectroscopy
  • Phosphatidylcholines with Interfered Masses of Protonated and Sodiated Molecules by Matrix-assisted Laser Desorption/Ionization Mass Spectroscopy (en)
skos:notation
  • RIV/00216275:25310/14:39899331!RIV15-MSM-25310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(EE2.3.30.0021), P(LL1302)
http://linked.open...iv/cisloPeriodika
  • září 2014
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 36459
http://linked.open...ai/riv/idVysledku
  • RIV/00216275:25310/14:39899331
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Mass Spectroscopy; Desorption/Ionization; Matrix-assisted Laser; Molecules; Protonated and Sodiated; Phosphatidylcholines (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [2B6798E97AC1]
http://linked.open...i/riv/nazevZdroje
  • Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 20
http://linked.open...iv/tvurceVysledku
  • Holčapek, Michal
  • Lísa, Miroslav
  • Chagovets, Vitaliy Viktorovich
issn
  • 1211-5541
number of pages
http://localhost/t...ganizacniJednotka
  • 25310
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