About: Long-term stability of parameters of antioxidant status in human serum

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://informahealthcare.com/doi/abs/10.3109/10715762.2013.797969
Description	The antioxidant status of serum or plasma can be determined using several commercially available assays. Here, four different assays, total antioxidant status (TAS), its second-generation assay (TAS2), biological antioxidant potential (BAP), and enzymatic assay using horseradish peroxidase (EAOC), were applied on human serum samples to test the temperature stability of antioxidants, upon storage of serum for 12 months. The two or three most commonly used temperatures for storage, that is, -20, -70 (or -80), and -196 degrees C, were selected. The general conclusion is that all assays were stable at the temperatures tested. In addition, there were almost no statistically significant differences between the samples stored at different temperatures. Only the rank order of the EAOC assay was not very good in samples stored at -20 degrees C. Also three components contributing to the total antioxidant capacity, uric acid, creatinine and bilirubin, showed no statistically significant differences between the temperatures. Therefore, storage at -20 degrees C is sufficient to maintain a proper assay outcome of most of the total antioxidant assays, although storage at -70/80 degrees C is to be preferred for longer storage times. The antioxidant status of serum or plasma can be determined using several commercially available assays. Here, four different assays, total antioxidant status (TAS), its second-generation assay (TAS2), biological antioxidant potential (BAP), and enzymatic assay using horseradish peroxidase (EAOC), were applied on human serum samples to test the temperature stability of antioxidants, upon storage of serum for 12 months. The two or three most commonly used temperatures for storage, that is, -20, -70 (or -80), and -196 degrees C, were selected. The general conclusion is that all assays were stable at the temperatures tested. In addition, there were almost no statistically significant differences between the samples stored at different temperatures. Only the rank order of the EAOC assay was not very good in samples stored at -20 degrees C. Also three components contributing to the total antioxidant capacity, uric acid, creatinine and bilirubin, showed no statistically significant differences between the temperatures. Therefore, storage at -20 degrees C is sufficient to maintain a proper assay outcome of most of the total antioxidant assays, although storage at -70/80 degrees C is to be preferred for longer storage times. (en)
Title	Long-term stability of parameters of antioxidant status in human serum Long-term stability of parameters of antioxidant status in human serum (en)
skos:prefLabel	Long-term stability of parameters of antioxidant status in human serum Long-term stability of parameters of antioxidant status in human serum (en)
skos:notation	RIV/00216275:25310/13:39896452!RIV14-MSM-25310___
http://linked.open...avai/predkladatel	Fakulta chemicko-technologická
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	6-7
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Mužáková, Vladimíra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	85411
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/13:39896452
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bilirubin; creatinine; uric acid; storage; total antioxidant status (en)
http://linked.open.../riv/klicoveSlovo	bilirubin storage uric acid total antioxidant status creatinine
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[4C760E9041A6]
http://linked.open...i/riv/nazevZdroje	Free Radical Research
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	47
http://linked.open...iv/tvurceVysledku	Skalický, J. Mužáková, Vladimíra Beekhof, P. K. Cremers, J. W. J. M. Jansen, E. H. J. M. Viezeliene, D.
http://linked.open...ain/vavai/riv/wos	000319945700011
issn	1071-5762
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.3109/10715762.2013.797969
http://localhost/t...ganizacniJednotka	25310

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