About: Amperometric Enzyme Carbon Paste-Based Biosensor for Quantification of Hydroquinone and Polyphenolic Antioxidant Capacity

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.electrochemsci.org/papers/vol8/80709030.pdf
Description	An amperometric biosensor for the determination of hydroquinone and other polyphenolic antioxidants is presented, based on a carbon paste electrode on which tyrosinase is immobilized in a Nafion (R) film. In a 0.1 M phosphate buffer (pH 7.0 and 25 +/- 1 degrees C), the antioxidant capacity can be expressed as the concentration equivalent of p-hydroquinone (HQ), according to an amperometric response of polyphenolic antioxidants with the carbon paste sensor at -0.24 V vs. Ag/AgCl/3 M KCl. In the range of low HQ concentrations (up to 120 mu mol L-1), the corresponding calibration curve follows the equation I [mu A] = -0.0426 -0.0113 c(HQ) [mu mol L-1] with a correlation coefficient R = 0.999. The limit of detection (LOD for S/N = 3) was estimated as 1.6 mu mol L-1, the repeatability was 1.2 % RSD (c = 90 mu M, n = 5 measurements). The biosensor can be applied to determine the phenolic antioxidant capacity in plants and food samples in the absence of higher concentrations of ascorbic acid. An amperometric biosensor for the determination of hydroquinone and other polyphenolic antioxidants is presented, based on a carbon paste electrode on which tyrosinase is immobilized in a Nafion (R) film. In a 0.1 M phosphate buffer (pH 7.0 and 25 +/- 1 degrees C), the antioxidant capacity can be expressed as the concentration equivalent of p-hydroquinone (HQ), according to an amperometric response of polyphenolic antioxidants with the carbon paste sensor at -0.24 V vs. Ag/AgCl/3 M KCl. In the range of low HQ concentrations (up to 120 mu mol L-1), the corresponding calibration curve follows the equation I [mu A] = -0.0426 -0.0113 c(HQ) [mu mol L-1] with a correlation coefficient R = 0.999. The limit of detection (LOD for S/N = 3) was estimated as 1.6 mu mol L-1, the repeatability was 1.2 % RSD (c = 90 mu M, n = 5 measurements). The biosensor can be applied to determine the phenolic antioxidant capacity in plants and food samples in the absence of higher concentrations of ascorbic acid. (en)
Title	Amperometric Enzyme Carbon Paste-Based Biosensor for Quantification of Hydroquinone and Polyphenolic Antioxidant Capacity Amperometric Enzyme Carbon Paste-Based Biosensor for Quantification of Hydroquinone and Polyphenolic Antioxidant Capacity (en)
skos:prefLabel	Amperometric Enzyme Carbon Paste-Based Biosensor for Quantification of Hydroquinone and Polyphenolic Antioxidant Capacity Amperometric Enzyme Carbon Paste-Based Biosensor for Quantification of Hydroquinone and Polyphenolic Antioxidant Capacity (en)
skos:notation	RIV/00216275:25310/13:39896383!RIV14-MSM-25310___
http://linked.open...avai/predkladatel	Fakulta chemicko-technologická
http://linked.open...avai/riv/aktivita	I S
http://linked.open...avai/riv/aktivity	I, S
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Sýs, Milan Vytřas, Karel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	60254
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/13:39896383
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	red wine analysis; hydroquinone; antioxidant capacity; tyrosinase; biosensor; amperometry; carbon paste electrode (en)
http://linked.open.../riv/klicoveSlovo	hydroquinone red wine analysis tyrosinase amperometry antioxidant capacity carbon paste electrode biosensor
http://linked.open...odStatuVydavatele	CS - Státní svaz Srbska a Černé Hory
http://linked.open...ontrolniKodProRIV	[553F074B537E]
http://linked.open...i/riv/nazevZdroje	International Journal of Electrochemical Science
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Kalcher, Kurt Vytřas, Karel Sýs, Milan Pekec, Bruna
http://linked.open...ain/vavai/riv/wos	000323547600010
issn	1452-3981
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	25310

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