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  • On-line two dimensional separation systems combining capillary liquid chromatography with capillary electrophoresis is presented, with special attention to the interface design. Two types of transparent interfaces have been fabricated in the poly methyl methacrylate, with different approaches to the transfer of the fractions from first dimension liquid chromatography separation to the second dimension micellar electrokinetic capillary chromatography separation. The interfaces used for the injection of the mobile phase effluent into the separation capillary are based either on the transverse flow of the background electrolyte, or on the voltage-switching in the tangentially connected channels. The application of both approaches for the separation of selected antioxidants is presented and merits of the interface designs are compared with the recently developed automated off-line twodimensional LCxMEKC separation method.
  • On-line two dimensional separation systems combining capillary liquid chromatography with capillary electrophoresis is presented, with special attention to the interface design. Two types of transparent interfaces have been fabricated in the poly methyl methacrylate, with different approaches to the transfer of the fractions from first dimension liquid chromatography separation to the second dimension micellar electrokinetic capillary chromatography separation. The interfaces used for the injection of the mobile phase effluent into the separation capillary are based either on the transverse flow of the background electrolyte, or on the voltage-switching in the tangentially connected channels. The application of both approaches for the separation of selected antioxidants is presented and merits of the interface designs are compared with the recently developed automated off-line twodimensional LCxMEKC separation method. (en)
Title
  • Fast On-line Two-dimensional LCxCE Using Flow and Voltage Gating Interfaces
  • Fast On-line Two-dimensional LCxCE Using Flow and Voltage Gating Interfaces (en)
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  • Fast On-line Two-dimensional LCxCE Using Flow and Voltage Gating Interfaces
  • Fast On-line Two-dimensional LCxCE Using Flow and Voltage Gating Interfaces (en)
skos:notation
  • RIV/00216275:25310/12:39895460!RIV13-GA0-25310___
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  • P(GAP206/12/0398)
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  • 136214
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  • RIV/00216275:25310/12:39895460
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  • capillary electrophoresis; capillary liquid chromatography; Two-dimensional separation (en)
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  • [81BEB15B6E52]
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  • Brno
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  • Brno
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  • CECE 2012: 9th International Interdisciplinary Meeting on Bioanalysis
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  • Fischer, Jan
  • Česla, Petr
  • Kovaříková, Lenka
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number of pages
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  • Ústav analytické chemie AV ČR, v. v. i.
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  • 978-80-904959-1-3
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  • 25310
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