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Description
  • The present work overviews the attempts at implementation of the conventional gradient theory developer for low-molecular compounds to the description and prediction of gradient separations of peptides and proteins on various types of HPLC columns: conventional analytical columns packed with wide-pore fully porous, fused-core superficially porous and non-porous particles; silica-based monolithic columns and organic-polymer poly(alkylmethacrylate) and poly(styrene-divinylbenzene) monolithic columns in capillary and disc formats. The attention is focused on the determination of the parameters necessary to predict gradient retention times (volumes) and bandwidths using the theoretical model equations. The accuracy of the prediction of protein retention on totally porous columns improves if size exclusion effect is taken into account, but this is not necessary with non-porous or superficially porous particles.
  • The present work overviews the attempts at implementation of the conventional gradient theory developer for low-molecular compounds to the description and prediction of gradient separations of peptides and proteins on various types of HPLC columns: conventional analytical columns packed with wide-pore fully porous, fused-core superficially porous and non-porous particles; silica-based monolithic columns and organic-polymer poly(alkylmethacrylate) and poly(styrene-divinylbenzene) monolithic columns in capillary and disc formats. The attention is focused on the determination of the parameters necessary to predict gradient retention times (volumes) and bandwidths using the theoretical model equations. The accuracy of the prediction of protein retention on totally porous columns improves if size exclusion effect is taken into account, but this is not necessary with non-porous or superficially porous particles. (en)
Title
  • Retention times and bandwidths in reversed-phase gradient liquid
  • Retention times and bandwidths in reversed-phase gradient liquid (en)
skos:prefLabel
  • Retention times and bandwidths in reversed-phase gradient liquid
  • Retention times and bandwidths in reversed-phase gradient liquid (en)
skos:notation
  • RIV/00216275:25310/11:39892628!RIV12-GA0-25310___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA203/07/0641), Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika
  • 49
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 226857
http://linked.open...ai/riv/idVysledku
  • RIV/00216275:25310/11:39892628
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Peptides; Proteins; Band compression; Bandwidth; Gradient elution (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [8CA3E1ED1F30]
http://linked.open...i/riv/nazevZdroje
  • Journal of Chromatography A
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 1218
http://linked.open...iv/tvurceVysledku
  • Jandera, Pavel
  • Kučerová, Zdeňka
  • Urban, Jiří
http://linked.open...ain/vavai/riv/wos
  • 000297776800014
http://linked.open...n/vavai/riv/zamer
issn
  • 0021-9673
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.chroma.2011.06.064
http://localhost/t...ganizacniJednotka
  • 25310
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