About: Effect of Selected Fungicides on Fusarium Growth and toxins production

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We examine the effects of selected fungicides on the Fusarium growth and toxins production especially of DON and T-2 toxins. Appropriate nutritive media were prepared containing different concentrations of the fungicides tested , inoculated with Fusarium moulds, and incubated under various conditions. The extraction of Fusarium toxins and their determination were carried out after the incubation using Agra Quant(R)Deoxynivalenol Test kit and Agra Quant(R) T-2 toxin Test kit. The results indicated a considerable variability of the individual strains in the formation of toxins. The strains of Fusarium graminearum CCM F-683 and Fusarium spp. produced large quantities of both DON and T-2 toxins. Fusarium poae CCM F-584 and Fusarium sporotrichioides CCM F-352 always produced larger quantities of only T-2 toxin in the given nutrient media. The experiment showed that the use of lower concentrations of fungicides resulted in an increased production of the monitored mycotoxins. The presence of Tri5 gene in the tested Fusarium strains was confirmed by PCR. The enzyme - trichodiene synthase - catalyses the isomerisation and cyclisation of farnesyl phosphate to trichodiene, the first step in the biosynthetic pathway of trichothecenes. We examine the effects of selected fungicides on the Fusarium growth and toxins production especially of DON and T-2 toxins. Appropriate nutritive media were prepared containing different concentrations of the fungicides tested , inoculated with Fusarium moulds, and incubated under various conditions. The extraction of Fusarium toxins and their determination were carried out after the incubation using Agra Quant(R)Deoxynivalenol Test kit and Agra Quant(R) T-2 toxin Test kit. The results indicated a considerable variability of the individual strains in the formation of toxins. The strains of Fusarium graminearum CCM F-683 and Fusarium spp. produced large quantities of both DON and T-2 toxins. Fusarium poae CCM F-584 and Fusarium sporotrichioides CCM F-352 always produced larger quantities of only T-2 toxin in the given nutrient media. The experiment showed that the use of lower concentrations of fungicides resulted in an increased production of the monitored mycotoxins. The presence of Tri5 gene in the tested Fusarium strains was confirmed by PCR. The enzyme - trichodiene synthase - catalyses the isomerisation and cyclisation of farnesyl phosphate to trichodiene, the first step in the biosynthetic pathway of trichothecenes. (en)
Title	Effect of Selected Fungicides on Fusarium Growth and toxins production Effect of Selected Fungicides on Fusarium Growth and toxins production (en)
skos:prefLabel	Effect of Selected Fungicides on Fusarium Growth and toxins production Effect of Selected Fungicides on Fusarium Growth and toxins production (en)
skos:notation	RIV/00216275:25310/11:39892302!RIV12-MSM-25310___
http://linked.open...avai/predkladatel	Fakulta chemicko-technologická
http://linked.open...avai/riv/aktivita	S Z
http://linked.open...avai/riv/aktivity	S, Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	SI
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Hrubošová, Dana Moťková, Petra Vytřasová, Jarmila
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	196443
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/11:39892302
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PCR; fungicide; T-2 toxin; deoxynivalenol; mycotoxin; genus Fusarium (en)
http://linked.open.../riv/klicoveSlovo	PCR T-2 toxin deoxynivalenol fungicide genus Fusarium mycotoxin
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[19424C14E663]
http://linked.open...i/riv/nazevZdroje	Czech journal of food sciences (Potravinářské vědy)
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Moťková, Petra Vytřasová, Jarmila Hrubošová, Dana
http://linked.open...ain/vavai/riv/wos	000299721700011
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	1212-1800
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	25310
is http://linked.open...avai/riv/vysledek of	Effect of Selected Fungicides on Fusarium Growth and toxins production Effect of Selected Fungicides on Fusarium Growth and toxins production

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