About: Voltammetric detection of ovalbumin at screen-printed electrodes in combination with immunomagnetic particles     Goto   Sponge   NotDistinct   Permalink

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  • Possibility of determination of ovalbumin as a model protein based on ELISA principle is reported in this contribution. Model system consisted of anti-ovalbumin IgG (primary antibody), ovalbumin and anti-ovalbumin-HRP (secondary antibody). After the substrate addition (hydrogen peroxide) current decrease induced by its conversion by HRP has been detected by linear sweep voltammetry. Superparamagnetic microspheres were used for covalent immobilization of primary antibodies which enables better antigen recognization compared to common sorption onto electrode surface.
  • Possibility of determination of ovalbumin as a model protein based on ELISA principle is reported in this contribution. Model system consisted of anti-ovalbumin IgG (primary antibody), ovalbumin and anti-ovalbumin-HRP (secondary antibody). After the substrate addition (hydrogen peroxide) current decrease induced by its conversion by HRP has been detected by linear sweep voltammetry. Superparamagnetic microspheres were used for covalent immobilization of primary antibodies which enables better antigen recognization compared to common sorption onto electrode surface. (en)
Title
  • Voltammetric detection of ovalbumin at screen-printed electrodes in combination with immunomagnetic particles
  • Voltammetric detection of ovalbumin at screen-printed electrodes in combination with immunomagnetic particles (en)
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  • Voltammetric detection of ovalbumin at screen-printed electrodes in combination with immunomagnetic particles
  • Voltammetric detection of ovalbumin at screen-printed electrodes in combination with immunomagnetic particles (en)
skos:notation
  • RIV/00216275:25310/11:39892179!RIV12-MSM-25310___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LC06035), S, Z(MSM0021627502)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
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http://linked.open...dnocenehoVysledku
  • 239509
http://linked.open...ai/riv/idVysledku
  • RIV/00216275:25310/11:39892179
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • ELISA; screen-printed electrodes; magnetic microspheres; ovalbumin; biosensor; voltammetric detection (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [00E45888AA06]
http://linked.open...v/mistoKonaniAkce
  • Pardubice
http://linked.open...i/riv/mistoVydani
  • Pardubice
http://linked.open...i/riv/nazevZdroje
  • Sensing in Electroanalysis Vol. 6
http://linked.open...in/vavai/riv/obor
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Metelka, Radovan
  • Šálek, Petr
  • Bílková, Zuzana
  • Korecká, Lucie
  • Čadková, Michaela
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
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  • Univerzita Pardubice
https://schema.org/isbn
  • 978-80-7395-434-5
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  • 25310
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