About: Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection

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Description	An HPLC method for the simultaneous measurement of phenylalanine and tyrosine in samples of dried blood spots and plasma has been developed and evaluated. We have used an inherent fluorescence of both phenylalanine and tyrosine. For the separation, a reverse-phase column LiChroCart 125-4, Purospher RP-18e, 5 um, was used. The mixture of ethanol and deionized water (5:95, v/v) was used as a mobile phase. Analytical performance of this method is satisfactory for both phenylalanine and tyrosine: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked plasma and blood samples were between 92.0 and 102.9%. The limit of detection was 10.0 and 5.0 umol/L, respectively. The preliminary reference ranges of phenylalanine and tyrosine in a group of newborns are 69.3 +/- 13.1 and 42.7 +/- 12.9 umol/L, in a group of blood donors are 68.4 +/- 9.9 and 52.1 +/- 10.9 umol/L. An HPLC method for the simultaneous measurement of phenylalanine and tyrosine in samples of dried blood spots and plasma has been developed and evaluated. We have used an inherent fluorescence of both phenylalanine and tyrosine. For the separation, a reverse-phase column LiChroCart 125-4, Purospher RP-18e, 5 um, was used. The mixture of ethanol and deionized water (5:95, v/v) was used as a mobile phase. Analytical performance of this method is satisfactory for both phenylalanine and tyrosine: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked plasma and blood samples were between 92.0 and 102.9%. The limit of detection was 10.0 and 5.0 umol/L, respectively. The preliminary reference ranges of phenylalanine and tyrosine in a group of newborns are 69.3 +/- 13.1 and 42.7 +/- 12.9 umol/L, in a group of blood donors are 68.4 +/- 9.9 and 52.1 +/- 10.9 umol/L. (en)
Title	Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection (en)
skos:prefLabel	Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection (en)
skos:notation	RIV/00216275:25310/09:00008563!RIV10-MSM-25310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	30
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Kanďár, Roman Žáková, Pavla
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	309926
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/09:00008563
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	dried blood spot; HPLC; fluorescence detection; N-methylphenylalanine; phenylalanine; phenylketonuria; tyrosine (en)
http://linked.open.../riv/klicoveSlovo	dried blood spot phenylketonuria N-methylphenylalanine HPLC phenylalanine tyrosine fluorescence detection
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[B26DC679308F]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography B
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	877
http://linked.open...iv/tvurceVysledku	Kanďár, Roman Žáková, Pavla
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	1570-0232
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	25310
is http://linked.open...avai/riv/vysledek of	Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection Determination of phenylalanine and tyrosine in plasma and dried blood samples using HPLC with fluorescence detection

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