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rdf:type
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Description
| - An amperometric method for salmonella detection was optimized. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (linked by alcaline phosphatase), froming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After substrate hydrolysis, the product formed (phenol) are detected amperometrically using a carbon paste electrode; current signals were monited at 0.65 V vs. Ag/AgCl/sat. KCl. A medium of pH 10 was found as the best. Amount of phenol generated bz SAAP conjugate was proportional to the number of Salmonella enteritidis in a sample
- An amperometric method for salmonella detection was optimized. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (linked by alcaline phosphatase), froming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After substrate hydrolysis, the product formed (phenol) are detected amperometrically using a carbon paste electrode; current signals were monited at 0.65 V vs. Ag/AgCl/sat. KCl. A medium of pH 10 was found as the best. Amount of phenol generated bz SAAP conjugate was proportional to the number of Salmonella enteritidis in a sample (en)
- V rámci výzkumu byly optimalizovány podmínky pro amperometrickou detekci buněk Salmonella s využitím uhlíkové pastové elektrody. Metoda je založena na reakci buněk salmonely se specifickou protilátkou značnou alkalickou fosfatázou. Po hydrolýze substrátu byl vzniklý fenol detekován amperometricky s využitím uhlíkové pastové elektrody. Signál proudu byl detekován při 0.65 V vs. Ag/AgCl/sat. KCl. nejvhodnějším prostedím pro detekci byl pufr s pH 10. Množství fenolu odpovídá počtu buněk Salmonella Enteritidis ve vzorku. (cs)
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Title
| - Optimizing of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrode
- Optimalizace metody amperometrické detekce Salmonella Enteritidis s využitím uhlíkové pastové elektrody (cs)
- Optimizing of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrode (en)
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skos:prefLabel
| - Optimizing of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrode
- Optimalizace metody amperometrické detekce Salmonella Enteritidis s využitím uhlíkové pastové elektrody (cs)
- Optimizing of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrode (en)
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skos:notation
| - RIV/00216275:25310/07:00006161!RIV08-MSM-25310___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(LC06035), Z(MSM0021627502)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/07:00006161
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Salmonella enteritidis; carbon paste electrode; amperometric detection; alkaline phosphatase (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
| - Pardubice, Česká republika
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Vytřasová, Jarmila
- Červenka, Libor
- Kristlová, Jana
- Pešková, Iva
- Hrubeš, Michal
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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number of pages
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http://purl.org/ne...btex#hasPublisher
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https://schema.org/isbn
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http://localhost/t...ganizacniJednotka
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is http://linked.open...avai/riv/vysledek
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