About: Extraction of DNA to detection of aflatoxigenic aspergillus species in foodstuffs using PCR method

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Extrakce DNA z aflatoxinogenních druhů aspergilů vyskytujících se v potravinách pro následnou polymerázovou řetězovou reakci. (cs) The extraction of DNA according to Cenis was used with different modifications of disrupting the cell wall. Fungi mycelium was disrupted by ultrasonic bath, Eppendorf homogenizer and liquid nitrogen. Method was optimized using pure cultures A. parasiticus var. globosus CCM F ? 550 and A. flavus CCM F ? 108. After extraction, acquired DNA was amplified by Polymerase Chain Reaction (PCR). PCR was used to amplify ver-1 gene and apa-2 gene as target fragments. The specific PCR product codes the biosynthetic way of aflatoxin B1. PCR product was detected by electrophoresis. Records were interpreted. Acquired results demonstrate, that success of method depends especially on disrupting cell wall of fungi. 91 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of the aflatoxinogenic fungi on the AFPA medium. Two samples contained two species fungi of genus Aspergillus and one sample contained three species fungi of genus Aspergillus. Finally w The extraction of DNA according to Cenis was used with different modifications of disrupting the cell wall. Fungi mycelium was disrupted by ultrasonic bath, Eppendorf homogenizer and liquid nitrogen. Method was optimized using pure cultures A. parasiticus var. globosus CCM F ? 550 and A. flavus CCM F ? 108. After extraction, acquired DNA was amplified by Polymerase Chain Reaction (PCR). PCR was used to amplify ver-1 gene and apa-2 gene as target fragments. The specific PCR product codes the biosynthetic way of aflatoxin B1. PCR product was detected by electrophoresis. Records were interpreted. Acquired results demonstrate, that success of method depends especially on disrupting cell wall of fungi. 91 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of the aflatoxinogenic fungi on the AFPA medium. Two samples contained two species fungi of genus Aspergillus and one sample contained three species fungi of genus Aspergillus. Finally w (en)
Title	Extraction of DNA to detection of aflatoxigenic aspergillus species in foodstuffs using PCR method Extraction of DNA to detection of aflatoxigenic aspergillus species in foodstuffs using PCR method (en) Extrakce DNA z aflatoxinogenních druhů aspergilů vyskytujících se v potravinách pro PCR (cs)
skos:prefLabel	Extraction of DNA to detection of aflatoxigenic aspergillus species in foodstuffs using PCR method Extraction of DNA to detection of aflatoxigenic aspergillus species in foodstuffs using PCR method (en) Extrakce DNA z aflatoxinogenních druhů aspergilů vyskytujících se v potravinách pro PCR (cs)
skos:notation	RIV/00216275:25310/07:00005935!RIV08-MSM-25310___
http://linked.open.../vavai/riv/strany	87?97
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA203/05/2106), P(LC06035), Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	13
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Moťková, Petra Vytřasová, Jarmila Brožková, Iveta
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	421473
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/07:00005935
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Aspergillus flavus; DNA extraction; PCR (en)
http://linked.open.../riv/klicoveSlovo	Aspergillus flavus PCR DNA extraction
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[27329A573851]
http://linked.open...i/riv/nazevZdroje	Scientific Papers of the University of Pardubice, Series A
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine. Modern instrumentation in chemical, food and clinical analysis
http://linked.open...UplatneniVysledku	2007
http://linked.open...iv/tvurceVysledku	Vytřasová, Jarmila Šnévajsová, Petra Brožková, Iveta
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	1211-5541
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	25310
is http://linked.open...avai/riv/vysledek of	Extraction of DNA to detection of aflatoxigenic aspergillus species in foodstuffs using PCR method Extraction of DNA to detection of aflatoxigenic aspergillus species in foodstuffs using PCR method Extraction of DNA to detection of aflatoxigenic aspergillus species in foodstuffs using PCR method

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