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Description
| - The isolation of proteins and peptides in the area of biosciences and biotechnology is now the subject of interest. Magnetic separation techniques have several advantages in comparison with standard separation procedures. Functional spherical nanoparticles with controlled tendency to aggregate and controlled level of nonspecific sorption could be focused in channel of magnetically active microfluidic device for isolation or enzyme modification of target protein directly in a microchannel. Biospecific ligand anhydrotrypsin enables highly specific trapping of peptides which correspond to the products generated by the action of various trypsin-like proteases and binds, in slightly acidic conditions, only specific peptides containing arginine and lysine residues at their C-termini [1]. Anhydrotrypsin is catalytically inactive derivative of bovine trypsin in which serine residue (Ser-195) is first modified with phenylmethanesulfonyl fluoride (PMSF), an active site-directed reagent, and then the modified
- The isolation of proteins and peptides in the area of biosciences and biotechnology is now the subject of interest. Magnetic separation techniques have several advantages in comparison with standard separation procedures. Functional spherical nanoparticles with controlled tendency to aggregate and controlled level of nonspecific sorption could be focused in channel of magnetically active microfluidic device for isolation or enzyme modification of target protein directly in a microchannel. Biospecific ligand anhydrotrypsin enables highly specific trapping of peptides which correspond to the products generated by the action of various trypsin-like proteases and binds, in slightly acidic conditions, only specific peptides containing arginine and lysine residues at their C-termini [1]. Anhydrotrypsin is catalytically inactive derivative of bovine trypsin in which serine residue (Ser-195) is first modified with phenylmethanesulfonyl fluoride (PMSF), an active site-directed reagent, and then the modified (en)
- Separace založená na biočipové technologii, specifická izolace tryptických peptidů pomocí anhydrotrypsinu. (cs)
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Title
| - Chip-based magnetic separation technique with immobilized trypsin and anhydrotrypsin for isolation of specific peptides of neurotensin
- Chip-based magnetic separation technique with immobilized trypsin and anhydrotrypsin for isolation of specific peptides of neurotensin (en)
- Separace založená na biočipové technologii, specifická izolace tryptických peptidů pomocí anhydrotrypsinu. (cs)
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skos:prefLabel
| - Chip-based magnetic separation technique with immobilized trypsin and anhydrotrypsin for isolation of specific peptides of neurotensin
- Chip-based magnetic separation technique with immobilized trypsin and anhydrotrypsin for isolation of specific peptides of neurotensin (en)
- Separace založená na biočipové technologii, specifická izolace tryptických peptidů pomocí anhydrotrypsinu. (cs)
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skos:notation
| - RIV/00216275:25310/05:00002852!RIV08-MSM-25310___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA203/05/2106), Z(MSM0021627502)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216275:25310/05:00002852
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - micro-chip; magnetic carrier; isolation; neurotensin (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - 11th International Symposium on Separation Science (ISSS 2005)
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Křenková, Jana
- Bílková, Zuzana
- Korecká, Lucie
- Slováková, Marcela
- Minc, Nicolas
- Viovy, Jean Louis
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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number of pages
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http://purl.org/ne...btex#hasPublisher
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https://schema.org/isbn
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http://localhost/t...ganizacniJednotka
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is http://linked.open...avai/riv/vysledek
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