About: TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S016950021400169X
Description	OBJECTIVES: Lung cancer with the ALK rearrangement constitutes only a small fraction of patients with non-small cell lung cancer (NSCLC). However, in the era of molecular-targeted therapy, efficient patient selection is crucial for successful treatment. In this context, an effective method for EML4-ALK detection is necessary. We developed a new highly sensitive variant specific TaqMan based real time PCR assay applicable to RNA from formalin-fixed paraffin-embedded tissue (FFPE). MATERIALS AND METHODS: This assay was used to analyze the EML4-ALK gene in 96 non-selected NSCLC specimens and compared with two other methods (end-point PCR and break-apart FISH). RESULTS: EML4-ALK was detected in 33/96 (34%) specimens using variant specific real time PCR, whereas in only 23/96 (24%) using end-point PCR. All real time PCR positive samples were confirmed with direct sequencing. A total of 46 specimens were subsequently analyzed by all three detection methods. OBJECTIVES: Lung cancer with the ALK rearrangement constitutes only a small fraction of patients with non-small cell lung cancer (NSCLC). However, in the era of molecular-targeted therapy, efficient patient selection is crucial for successful treatment. In this context, an effective method for EML4-ALK detection is necessary. We developed a new highly sensitive variant specific TaqMan based real time PCR assay applicable to RNA from formalin-fixed paraffin-embedded tissue (FFPE). MATERIALS AND METHODS: This assay was used to analyze the EML4-ALK gene in 96 non-selected NSCLC specimens and compared with two other methods (end-point PCR and break-apart FISH). RESULTS: EML4-ALK was detected in 33/96 (34%) specimens using variant specific real time PCR, whereas in only 23/96 (24%) using end-point PCR. All real time PCR positive samples were confirmed with direct sequencing. A total of 46 specimens were subsequently analyzed by all three detection methods. (en)
Title	TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC (en)
skos:prefLabel	TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC (en)
skos:notation	RIV/00216224:14740/14:00077719!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(EE2.3.20.0045)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Lišková, Květoslava Skřičková, Jana Tomíšková, Marcela Pospíšilová, Šárka Dvořáková, Dana Mayer, Jiří Robešová, Blanka Bajerová, Monika
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	49307
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00077719
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Lung cancer; Targeted therapy; NSCLC; EML4-ALK; Real time PCR; FISH (en)
http://linked.open.../riv/klicoveSlovo	Targeted therapy FISH Real time PCR Lung cancer NSCLC EML4-ALK
http://linked.open...odStatuVydavatele	IE - Irsko
http://linked.open...ontrolniKodProRIV	[159502C0DDD7]
http://linked.open...i/riv/nazevZdroje	Lung Cancer
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology Support of Professional Growth and International Integration of Research Teams in the Filed of Molecular Medicine
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	85
http://linked.open...iv/tvurceVysledku	Dvořáková, Dana Mayer, Jiří Pospíšilová, Šárka Skřičková, Jana Tomíšková, Marcela Bajerová, Monika Robešová, Blanka Lišková, Květoslava
http://linked.open...ain/vavai/riv/wos	000338617100005
issn	0169-5002
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.lungcan.2014.04.002
http://localhost/t...ganizacniJednotka	14740

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