About: Recombinant Lectin from insect pathogen Photorhabdus luminisnce: structural and functional studies

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Photorhabdus luminescence is Gram-negative bacilli, exhibit duel nature of lifecycle one, being pathogenic towards insect whereas other, symbiosis with entamo-pathogenic nematodes. The lectin gene was amplified from the genome of P. luminescence and cloned with polyhistidine tag. Expression and production of the lectin was optimized and further purified it using nickel affinity chromatography. The lectin was found to be tetramer of 160 kDa exhibiting 41 kDa monomers interconnected with disulphide bridge. Crystallization of the lectin was achieved after various screening kits and optimized further by hanging drop method. The crystal structure was solved at 1.8 A resolution. The lectin was found to be seven-bladed beta-propeller fold with possibly two different binding sites per monomer. One of the binding site was found to be fucose specific however other was also suggested to be saccharide specificity probably towards a saccharide with higher level of hydroxylation than L-fucopyranose. Photorhabdus luminescence is Gram-negative bacilli, exhibit duel nature of lifecycle one, being pathogenic towards insect whereas other, symbiosis with entamo-pathogenic nematodes. The lectin gene was amplified from the genome of P. luminescence and cloned with polyhistidine tag. Expression and production of the lectin was optimized and further purified it using nickel affinity chromatography. The lectin was found to be tetramer of 160 kDa exhibiting 41 kDa monomers interconnected with disulphide bridge. Crystallization of the lectin was achieved after various screening kits and optimized further by hanging drop method. The crystal structure was solved at 1.8 A resolution. The lectin was found to be seven-bladed beta-propeller fold with possibly two different binding sites per monomer. One of the binding site was found to be fucose specific however other was also suggested to be saccharide specificity probably towards a saccharide with higher level of hydroxylation than L-fucopyranose. (en)
Title	Recombinant Lectin from insect pathogen Photorhabdus luminisnce: structural and functional studies Recombinant Lectin from insect pathogen Photorhabdus luminisnce: structural and functional studies (en)
skos:prefLabel	Recombinant Lectin from insect pathogen Photorhabdus luminisnce: structural and functional studies Recombinant Lectin from insect pathogen Photorhabdus luminisnce: structural and functional studies (en)
skos:notation	RIV/00216224:14740/14:00074228!RIV15-GA0-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA13-25401S)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Rozumová, Petra Demo, Gabriel Wimmerová, Michaela Kumar, Atul
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	41613
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00074228
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Lectin Insect pathogen Photorhabdus luminisnce (en)
http://linked.open.../riv/klicoveSlovo	Lectin Insect pathogen Photorhabdus luminisnce
http://linked.open...ontrolniKodProRIV	[4F32FA938EE5]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of proteins from pathogens involved in host organism recognition
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Demo, Gabriel Wimmerová, Michaela Sýkorová, Petra Kumar, Atul
http://localhost/t...ganizacniJednotka	14740

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