About: Determination of sterols using liquid chromatography with off-line surface-assisted laser desorption/ionization mass spectrometry

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About: Determination of sterols using liquid chromatography with off-line surface-assisted laser desorption/ionization mass spectrometry Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S0021967314010206
Description	A new method, reversed phase liquid chromatography with off-line surface-assisted laser desorption/ionization mass spectrometry (RPLC–SALDI MS) for the determination of brassicasterol (BR), cholesterol (CH), stigmasterol (ST), campesterol (CA) and b-sitosterol (SI) in oil samples has been developed. The sample preparation consisted of alkaline saponification followed by extraction of the unsaponificable fraction with diethyl ether. The recovery of the sterols ranged from 91 to 95% with RSD less than 4%. Separation of the five major sterols on a C18 column using methanol-water gradient was achieved in about 10 min. An on-line UV detector was employed for the initial sterol detection prior to effluent deposition using a laboratory-built spotter with 1:73 splitter. Off-line SALDI MS was then applied for mass determination/identification and quantification of the separated sterols. A new method, reversed phase liquid chromatography with off-line surface-assisted laser desorption/ionization mass spectrometry (RPLC–SALDI MS) for the determination of brassicasterol (BR), cholesterol (CH), stigmasterol (ST), campesterol (CA) and b-sitosterol (SI) in oil samples has been developed. The sample preparation consisted of alkaline saponification followed by extraction of the unsaponificable fraction with diethyl ether. The recovery of the sterols ranged from 91 to 95% with RSD less than 4%. Separation of the five major sterols on a C18 column using methanol-water gradient was achieved in about 10 min. An on-line UV detector was employed for the initial sterol detection prior to effluent deposition using a laboratory-built spotter with 1:73 splitter. Off-line SALDI MS was then applied for mass determination/identification and quantification of the separated sterols. (en)
Title	Determination of sterols using liquid chromatography with off-line surface-assisted laser desorption/ionization mass spectrometry Determination of sterols using liquid chromatography with off-line surface-assisted laser desorption/ionization mass spectrometry (en)
skos:prefLabel	Determination of sterols using liquid chromatography with off-line surface-assisted laser desorption/ionization mass spectrometry Determination of sterols using liquid chromatography with off-line surface-assisted laser desorption/ionization mass spectrometry (en)
skos:notation	RIV/00216224:14740/14:00073825!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(GAP206/12/0538), S
http://linked.open...iv/cisloPeriodika	5 September
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Roblová, Vendula Preisler, Jan Vrbková, Blanka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	10824
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00073825
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	sterols; SALDI MS; HPLC (en)
http://linked.open.../riv/klicoveSlovo	sterols SALDI MS HPLC
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[802800796F6D]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Novel front-end strategies for desorption mass spectrometry Central european institute of technology
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	1358
http://linked.open...iv/tvurceVysledku	Preisler, Jan Roblová, Vendula Vrbková, Blanka Yeung, Edward S.
http://linked.open...ain/vavai/riv/wos	000340979200013
issn	0021-9673
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.chroma.2014.06.077
http://localhost/t...ganizacniJednotka	14740

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