About: A simplified method for peptide de novo sequencing using O-18 labeling

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About: A simplified method for peptide de novo sequencing using O-18 labeling Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	https://www.impublications.com/content/abstract?code=E20_0255
Description	Incorporation of an O-18 atom into a peptide C-terminus by proteolytic cleavage in the presence of (H2O)-O-18 is one of the most effective ways of enhancing tandem mass spectrometry (MS/MS)-based de novo sequencing. Incorporation is usually accomplished by procedures including vacuum-assisted drying of tryptic peptides extracted from gels, their subsequent reconstitution in a (H2O)-O-16/(H2O)-O-18 mixture and re-treatment with trypsin. In the present work, we propose a simplified procedure for O-18 incorporation into tryptic peptides by adding (H2O)-O-18 and trypsin to the original digest solution. In comparison to published methods, the proposed protocol for peptide de novo sequencing brings significant advantages in analysis and workflow with no deterioration in method performance. Incorporation of an O-18 atom into a peptide C-terminus by proteolytic cleavage in the presence of (H2O)-O-18 is one of the most effective ways of enhancing tandem mass spectrometry (MS/MS)-based de novo sequencing. Incorporation is usually accomplished by procedures including vacuum-assisted drying of tryptic peptides extracted from gels, their subsequent reconstitution in a (H2O)-O-16/(H2O)-O-18 mixture and re-treatment with trypsin. In the present work, we propose a simplified procedure for O-18 incorporation into tryptic peptides by adding (H2O)-O-18 and trypsin to the original digest solution. In comparison to published methods, the proposed protocol for peptide de novo sequencing brings significant advantages in analysis and workflow with no deterioration in method performance. (en)
Title	A simplified method for peptide de novo sequencing using O-18 labeling A simplified method for peptide de novo sequencing using O-18 labeling (en)
skos:prefLabel	A simplified method for peptide de novo sequencing using O-18 labeling A simplified method for peptide de novo sequencing using O-18 labeling (en)
skos:notation	RIV/00216224:14740/14:00073785!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(GBP206/12/G151), S
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Zdráhal, Zbyněk Šedo, Ondrej Havliš, Jan Voráč, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	1176
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00073785
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	peptide de novo sequencing; mass spectrometry; isotopic labeling; O-18 incorporation (en)
http://linked.open.../riv/klicoveSlovo	isotopic labeling mass spectrometry O-18 incorporation peptide de novo sequencing
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[B361ACA8B107]
http://linked.open...i/riv/nazevZdroje	European Journal of Mass Spectrometry
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of novel approaches to bioanalysis and molecular diagnostics Central european institute of technology
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	20
http://linked.open...iv/tvurceVysledku	Zdráhal, Zbyněk Šedo, Ondrej Havliš, Jan Voráč, Aleš
http://linked.open...ain/vavai/riv/wos	000337268400006
issn	1469-0667
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1255/ejms.1277
http://localhost/t...ganizacniJednotka	14740

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