About: Spectral density mapping protocols for analysis of molecular motions in disordered proteins

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/article/10.1007%2Fs10858-014-9816-4
Description	Spectral density mapping represents the method of choice for investigations of molecular motions of intrinsically disordered proteins (IDPs). However, the current methodology has been developed for well-folded proteins. In order to find conditions for a reliable analysis of relaxation of IDPs, accuracy of the current reduced spectral density mapping protocols applied to IDPs was examined and new spectral density mapping methods employing cross-correlated relaxation rates have been designed. Various sources of possible systematic errors were analyzed theoretically and the presented approaches were tested on a partially disordered protein, delta subunit of bacterial RNA polymerase. Results showed that the proposed protocols provide unbiased description of molecular motions of IDPs and allow to separate slow exchange from fast dynamics. Spectral density mapping represents the method of choice for investigations of molecular motions of intrinsically disordered proteins (IDPs). However, the current methodology has been developed for well-folded proteins. In order to find conditions for a reliable analysis of relaxation of IDPs, accuracy of the current reduced spectral density mapping protocols applied to IDPs was examined and new spectral density mapping methods employing cross-correlated relaxation rates have been designed. Various sources of possible systematic errors were analyzed theoretically and the presented approaches were tested on a partially disordered protein, delta subunit of bacterial RNA polymerase. Results showed that the proposed protocols provide unbiased description of molecular motions of IDPs and allow to separate slow exchange from fast dynamics. (en)
Title	Spectral density mapping protocols for analysis of molecular motions in disordered proteins Spectral density mapping protocols for analysis of molecular motions in disordered proteins (en)
skos:prefLabel	Spectral density mapping protocols for analysis of molecular motions in disordered proteins Spectral density mapping protocols for analysis of molecular motions in disordered proteins (en)
skos:notation	RIV/00216224:14740/14:00073529!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.1.00/02.0068), P(GA13-16842S)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Sklenář, Vladimír Kadeřávek, Pavel Žídek, Lukáš Zapletal, Vojtěch
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	46558
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00073529
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Nuclear magnecit resonance; Relaxation; Spectral density function; Intrinsically disordered proteins (en)
http://linked.open.../riv/klicoveSlovo	Intrinsically disordered proteins Relaxation Nuclear magnecit resonance Spectral density function
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[9F8C450EAC33]
http://linked.open...i/riv/nazevZdroje	Journal of Biomolecular NMR
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	PODJEDNOTKY URČUJÍCÍ DNA SPECIFICITU BAKTERIÁLNÍ RNA POLYMERÁZY S FLEXIBILNÍMI DOMÉNAMI: FUNKCE A DYNAMIKA Central european institute of technology
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	58
http://linked.open...iv/tvurceVysledku	Kadeřávek, Pavel Krásný, Libor Sklenář, Vladimír Žídek, Lukáš Rabatinová, Alžběta Zapletal, Vojtěch
http://linked.open...ain/vavai/riv/wos	000332743800005
issn	0925-2738
number of pages	15 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10858-014-9816-4
http://localhost/t...ganizacniJednotka	14740

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