About: Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	DNA methylation is an important epigenetic event playing crucial roles in physiologic and pathologic processes. We show that methylation of cytosine (C) residues in DNA can be easily detected electrochemically using mercury or solid amalgam electrodes. Reduction peaks of untreated single-stranded methylated and non-methylated oligodeoxynucleotides (ODN) do not significantly differ. Using DNA bisulfite treatment, reducible Cs are transformed into nonreducible uracil residues, strongly decreasing square wave voltammetric C reduction peaks. On the other hand. 5-methylcytosine (mC) residues resist the bisulfite treatment and display almost unchanged reduction peak. Desulfonation step should be omitted because uracil sulfonation improves the resolution of C from mC. By combining DNA bisulfite treatment with square wave voltammetry. DNA methylation can be determined quantitatively at nanomolar and subnamolar ODN concentrations. DNA methylation is an important epigenetic event playing crucial roles in physiologic and pathologic processes. We show that methylation of cytosine (C) residues in DNA can be easily detected electrochemically using mercury or solid amalgam electrodes. Reduction peaks of untreated single-stranded methylated and non-methylated oligodeoxynucleotides (ODN) do not significantly differ. Using DNA bisulfite treatment, reducible Cs are transformed into nonreducible uracil residues, strongly decreasing square wave voltammetric C reduction peaks. On the other hand. 5-methylcytosine (mC) residues resist the bisulfite treatment and display almost unchanged reduction peak. Desulfonation step should be omitted because uracil sulfonation improves the resolution of C from mC. By combining DNA bisulfite treatment with square wave voltammetry. DNA methylation can be determined quantitatively at nanomolar and subnamolar ODN concentrations. (en)
Title	Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA (en)
skos:prefLabel	Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA (en)
skos:notation	RIV/00216224:14740/12:00073027!RIV14-MSM-14740___
http://linked.open...avai/predkladatel	Středoevropský technologický institut
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(ED2.1.00/03.0101), P(GAP301/11/2055), P(ME09038), Z(AV0Z50040702)
http://linked.open...iv/cisloPeriodika	Sep
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Fojta, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	133865
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/12:00073027
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA methylation; 5-Methylcytosine analysis; Sodium bisulfite; Mercury electrode; Solid amalgam electrode (en)
http://linked.open.../riv/klicoveSlovo	Mercury electrode 5-Methylcytosine analysis Sodium bisulfite Solid amalgam electrode DNA methylation
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[E90F253B820E]
http://linked.open...i/riv/nazevZdroje	Electrochimica Acta
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Regional Centre for Applied Molecular Oncology (RECAMO) New methods of analysis of proteins and their glycosylation in cancer - combination of electrochemistry, microfludic biosensors and mass spectrometry. Interactions of proteins and nucleic acids with surfaces. New tools for biomedicine
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	78
http://linked.open...iv/tvurceVysledku	Fojta, Miroslav Bartosik, Martin Palecek, Emil
http://linked.open...ain/vavai/riv/wos	000308259500010
http://linked.open...n/vavai/riv/zamer	Genome and epigenome: 1D and 3D Structure, Dynamics, Interactions with Proteins and Functions
issn	0013-4686
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.electacta.2012.05.115
http://localhost/t...ganizacniJednotka	14740

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software