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  • Direct matrix-assisted laser desorption/ionization mass spec- trometry (MALDI-MS) bacterial cell or lysate analysis appears to meet all the criteria required for a rapid and reliable analytical microorganism identification and taxonomical classification tool. Few-minute analytical procedure providing information extending up to sub-species level underlines the potential of the MALDI-MS profiling in comparison with other methods employed in the field. However, the quality of MALDI-MS profiles and consequently the performance of the method are influenced by numerous factors, which involve particular steps of the sample preparation procedure. This review is aimed at advances in development and optimization of the MALDI-MS profiling methodology. Approaches improving the quality of the MALDI-MS profiles and universal feasibility of the method are discussed.
  • Direct matrix-assisted laser desorption/ionization mass spec- trometry (MALDI-MS) bacterial cell or lysate analysis appears to meet all the criteria required for a rapid and reliable analytical microorganism identification and taxonomical classification tool. Few-minute analytical procedure providing information extending up to sub-species level underlines the potential of the MALDI-MS profiling in comparison with other methods employed in the field. However, the quality of MALDI-MS profiles and consequently the performance of the method are influenced by numerous factors, which involve particular steps of the sample preparation procedure. This review is aimed at advances in development and optimization of the MALDI-MS profiling methodology. Approaches improving the quality of the MALDI-MS profiles and universal feasibility of the method are discussed. (en)
Title
  • Sample preparation methods for MALDI-MS profiling of bacteria
  • Sample preparation methods for MALDI-MS profiling of bacteria (en)
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  • Sample preparation methods for MALDI-MS profiling of bacteria
  • Sample preparation methods for MALDI-MS profiling of bacteria (en)
skos:notation
  • RIV/00216224:14740/11:00052198!RIV12-MSM-14740___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LC06034), Z(MSM0021622415), Z(MSM0021622416)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 228123
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14740/11:00052198
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • MALDI-MS; bacteria profiling; microorganism identification (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [AC38B7B0E7F4]
http://linked.open...i/riv/nazevZdroje
  • Mass Spectrometry Reviews
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http://linked.open...vavai/riv/projekt
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http://linked.open...v/svazekPeriodika
  • 30
http://linked.open...iv/tvurceVysledku
  • Sedláček, Ivo
  • Zdráhal, Zbyněk
  • Šedo, Ondrej
http://linked.open...ain/vavai/riv/wos
  • 000289532100004
http://linked.open...n/vavai/riv/zamer
issn
  • 0277-7037
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/mas.20287
http://localhost/t...ganizacniJednotka
  • 14740
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