About: Meiosis and sporulation in fission yeast: the effect of cytoskeletal inhibitors

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Homothallic yeasts Schizosaccharomyces japonicus var. versatilis, undergo mass mating, meiosis and sporulation when they reach stationary phase after growth in malt extract broth glucose medium. Microtubular inhibitors benomyl (BEM), thiabendazole (TBZ) and methylbenzimidazole (MBC) were applied in concentrations 10 -150 mikro g/ml at different phases of culture growth and their cytological effects were followed microscopically. MBC inhibited the increase of cell number at 200 mikro g/ml and induced cytolysis. BEM disintegrated microtubules completely at 30 mikro g/ml. Lower concentrations of inhibitor (5 -20 mikro g/ml) disturbed sporulation -asci with 1 to 7 spore appeared instead of regular 8 spores per ascus. Frequently the sporulation process turned out abortive. TBZ affected the growth curve from concentration 30 mikro g/ml, where part of the cell population showed the microtubules partly disrupted. The microtubules disappeared completely at 50 mikro g/ml. Mating projections were formed at 30 o Homothallic yeasts Schizosaccharomyces japonicus var. versatilis, undergo mass mating, meiosis and sporulation when they reach stationary phase after growth in malt extract broth glucose medium. Microtubular inhibitors benomyl (BEM), thiabendazole (TBZ) and methylbenzimidazole (MBC) were applied in concentrations 10 -150 mikro g/ml at different phases of culture growth and their cytological effects were followed microscopically. MBC inhibited the increase of cell number at 200 mikro g/ml and induced cytolysis. BEM disintegrated microtubules completely at 30 mikro g/ml. Lower concentrations of inhibitor (5 -20 mikro g/ml) disturbed sporulation -asci with 1 to 7 spore appeared instead of regular 8 spores per ascus. Frequently the sporulation process turned out abortive. TBZ affected the growth curve from concentration 30 mikro g/ml, where part of the cell population showed the microtubules partly disrupted. The microtubules disappeared completely at 50 mikro g/ml. Mating projections were formed at 30 o (en)
Title	Meiosis and sporulation in fission yeast: the effect of cytoskeletal inhibitors Meiosis and sporulation in fission yeast: the effect of cytoskeletal inhibitors (en)
skos:prefLabel	Meiosis and sporulation in fission yeast: the effect of cytoskeletal inhibitors Meiosis and sporulation in fission yeast: the effect of cytoskeletal inhibitors (en)
skos:notation	RIV/00216224:14330/99:00003926!RIV/2002/GA0/143302/N
http://linked.open.../vavai/riv/strany	70
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA204/97/1150)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Holubářová, Alena Svoboda, Augustin
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	745542
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/99:00003926
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Meiosis and sporulation in fission yeast: the effect of cytoskeletal inhibitors (en)
http://linked.open.../riv/klicoveSlovo	Meiosis and sporulation in fission yeast: the effect of cytoskeletal inhibitors
http://linked.open...ontrolniKodProRIV	[C0CE82D4F038]
http://linked.open...v/mistoKonaniAkce	Bratislava
http://linked.open...i/riv/mistoVydani	Bratislava
http://linked.open...i/riv/nazevZdroje	20 th International Specialized Symposium on Yeast 1999
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	The cell wall and morphogenesis in yeast cells: a role for the cytoskeleton
http://linked.open...UplatneniVysledku	1999
http://linked.open...iv/tvurceVysledku	Svoboda, Augustin Holubářová, Alena
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	1999-01-01 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Czech and Slovak Yeast Commission of the Czechoslovak Society for Mikrobiology
http://localhost/t...ganizacniJednotka	14330

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