About: Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods

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About: Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Preclinical studies have demonstrated the promising potential of human induced pluripotent stem cells (hiPSCs) for clinical application. To fulfill this goal, efficient and safe methods to generate them must be established. Various reprogramming techniques were presented during 7 years of hiPSCs research. Genome non-integrating and completely xeno-free protocols from the first biopsy to stable hiPSCs clones are highly preferable in terms of future clinical application. We successfully generated hiPSCs using STEMCCA lentivirus, Sendai virus or episomal vectors. Human fibroblasts and CD34+ blood progenitors were used as a source cells and were maintained either on mouse embryonic feeder cells or in feeder-free conditions. The reprogramming efficiency was comparable for all three methods and both cell types, while the best results were obtained in feeder-free conditions. Preclinical studies have demonstrated the promising potential of human induced pluripotent stem cells (hiPSCs) for clinical application. To fulfill this goal, efficient and safe methods to generate them must be established. Various reprogramming techniques were presented during 7 years of hiPSCs research. Genome non-integrating and completely xeno-free protocols from the first biopsy to stable hiPSCs clones are highly preferable in terms of future clinical application. We successfully generated hiPSCs using STEMCCA lentivirus, Sendai virus or episomal vectors. Human fibroblasts and CD34+ blood progenitors were used as a source cells and were maintained either on mouse embryonic feeder cells or in feeder-free conditions. The reprogramming efficiency was comparable for all three methods and both cell types, while the best results were obtained in feeder-free conditions. (en)
Title	Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods (en)
skos:prefLabel	Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods Generation of Human Induced Pluripotent Stem Cells Using Genome Integrating or Non-integrating Methods (en)
skos:notation	RIV/00216224:14330/14:00074082!RIV15-GA0-14330___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GBP302/12/G157)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Paďourová, Stanislava Šimara, Pavel Tesařová, Lenka
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	18059
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/14:00074082
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	hiPSCs; lentivirus; Sendai virus; episomal reprogramming (en)
http://linked.open.../riv/klicoveSlovo	Sendai virus episomal reprogramming hiPSCs lentivirus
http://linked.open...ontrolniKodProRIV	[65AD8A1D5E6E]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Šimara, Pavel Krontorád Koutná, Irena Tesařová, Lenka Paďourová, Stanislava
http://localhost/t...ganizacniJednotka	14330

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