About: Interaction of endonuclease G with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET imaging of living cells

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About: Interaction of endonuclease G with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET imaging of living cells Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Apoptosis is a natural form of cell death involved in many physiological changes in the cell. During some forms of cell death, proteins endonuclease G (EndoG) and apoptosis-inducing factor (AIF) are released from mitochondria, then they translocate into the cell nuclei, where they participate in chromatin degradation in a caspase-independent way. We have conducted living-cell confocal fluorescence microscopy followed by analysis of fluorescence resonance energy transfer (FRET) to observe the protein interaction of EndoG with AIF and their interactions with other proteins in human cell nuclei after induction of apoptosis. Our results show that EndoG interacts with histone H2B, AIF, and DNA topoisomerase II alpha (TOPO2a). Also AIF was found to interact with TOPO2a. Therefore we can conclude that EndoG, AIF, and TOPO2a may form a protein complex allowing chromatin degradation in apoptotic nucleus. Apoptosis is a natural form of cell death involved in many physiological changes in the cell. During some forms of cell death, proteins endonuclease G (EndoG) and apoptosis-inducing factor (AIF) are released from mitochondria, then they translocate into the cell nuclei, where they participate in chromatin degradation in a caspase-independent way. We have conducted living-cell confocal fluorescence microscopy followed by analysis of fluorescence resonance energy transfer (FRET) to observe the protein interaction of EndoG with AIF and their interactions with other proteins in human cell nuclei after induction of apoptosis. Our results show that EndoG interacts with histone H2B, AIF, and DNA topoisomerase II alpha (TOPO2a). Also AIF was found to interact with TOPO2a. Therefore we can conclude that EndoG, AIF, and TOPO2a may form a protein complex allowing chromatin degradation in apoptotic nucleus. (en)
Title	Interaction of endonuclease G with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET imaging of living cells Interaction of endonuclease G with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET imaging of living cells (en)
skos:prefLabel	Interaction of endonuclease G with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET imaging of living cells Interaction of endonuclease G with histone H2B, AIF, and DNA topoisomerase II alpha during apoptosis as revealed by FRET imaging of living cells (en)
skos:notation	RIV/00216224:14330/11:00053487!RIV12-MSM-14330___
http://linked.open...avai/predkladatel	Fakulta informatiky
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06052), P(LC535), Z(MSM0021622419)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Vařecha, Miroslav Kozubek, Michal Potěšilová, Michaela Matula, Pavel
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	205233
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/11:00053487
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	endonuclease G; DNA topoisomerase II alpha; histone H2B; apoptosis-inducing factor; FRET; apoptosis (en)
http://linked.open.../riv/klicoveSlovo	apoptosis-inducing factor endonuclease G DNA topoisomerase II alpha FRET histone H2B apoptosis
http://linked.open...ontrolniKodProRIV	[DE566C723CA2]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Matula, Pavel Kozubek, Michal Potěšilová, Michaela Vařecha, Miroslav
http://linked.open...n/vavai/riv/zamer	Highly Parallel and Distributed Computing Systems
http://localhost/t...ganizacniJednotka	14330

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