About: Fast Tracking Algorithm of GFP-Transfected Living Cells Based on the Chan-Vese Model     Goto   Sponge   NotDistinct   Permalink

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  • We present a general, robust, and fast approach for tracking GFP-transfected living cells in time-lapse series acquired using a confocal fluorescence microscope. The proposed tracking scheme involves two steps. First, the coherence-enhancing diffusion filtering is applied on each frame in order to reduce the noise and enhance flow-like structures. Second, enhanced cell boundaries are detected through a minimization of the Chan-Vese model that divides an image domain into two possibly disconnected regions of minimal variance. To speed up the second step, final contours from the previous frame are taken as seeds in the next one. The minimization of the Chan-Vese model is implemented using a fast level set-like algorithm (Maška et al. 2010) achieving near real-time performance in 2D.
  • We present a general, robust, and fast approach for tracking GFP-transfected living cells in time-lapse series acquired using a confocal fluorescence microscope. The proposed tracking scheme involves two steps. First, the coherence-enhancing diffusion filtering is applied on each frame in order to reduce the noise and enhance flow-like structures. Second, enhanced cell boundaries are detected through a minimization of the Chan-Vese model that divides an image domain into two possibly disconnected regions of minimal variance. To speed up the second step, final contours from the previous frame are taken as seeds in the next one. The minimization of the Chan-Vese model is implemented using a fast level set-like algorithm (Maška et al. 2010) achieving near real-time performance in 2D. (en)
Title
  • Fast Tracking Algorithm of GFP-Transfected Living Cells Based on the Chan-Vese Model
  • Fast Tracking Algorithm of GFP-Transfected Living Cells Based on the Chan-Vese Model (en)
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  • Fast Tracking Algorithm of GFP-Transfected Living Cells Based on the Chan-Vese Model
  • Fast Tracking Algorithm of GFP-Transfected Living Cells Based on the Chan-Vese Model (en)
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  • RIV/00216224:14330/11:00051908!RIV12-MSM-14330___
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  • P(2B06052), P(LC535), S, Z(MSM0021622419)
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  • 199374
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  • RIV/00216224:14330/11:00051908
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  • object tracking; Chan-Vese model; level set framework; coherence-enhancing diffusion (en)
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  • [1BA609D14D07]
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  • Matula, Pavel
  • Maška, Martin
  • Muñoz-Barrutia, Arrate
  • Ortiz-de-Solórzano, Carlos
http://linked.open...n/vavai/riv/zamer
http://localhost/t...ganizacniJednotka
  • 14330
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