About: Interactions of apoptotic proteins AIF and endonuclease G analyzed by bioinformatic predictions, molecular docking, and fluorescent microscopy

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About: Interactions of apoptotic proteins AIF and endonuclease G analyzed by bioinformatic predictions, molecular docking, and fluorescent microscopy Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	During apoptosis several mitochondrial proteins are released. Some of them participate in caspase-independent nuclear DNA degradation, especially apoptosis-inducing factor (AIF) and endonuclease G (endoG). We studied the structure, cellular localization, and interactions of several proteins in silico and in vitro using fluorescent microscopy. Bioinformatic predictions were conducted to analyze the presence of interaction sites in the studied proteins. We conducted molecular modeling of proteins with unknown 3D structure: endonuclease G, CPS-6, WAH-1, and heat shock protein 70-1. These models were then refined by MolProbity server and employed together with experimentally known 3D structures of other proteins like AIF and cyclophilin A in molecular docking simulations of interactions. Fluorescence resonance energy transfer (FRET) technique and consequent image analysis was used to evaluate the interactions of fluorescently labeled proteins in cells. During apoptosis several mitochondrial proteins are released. Some of them participate in caspase-independent nuclear DNA degradation, especially apoptosis-inducing factor (AIF) and endonuclease G (endoG). We studied the structure, cellular localization, and interactions of several proteins in silico and in vitro using fluorescent microscopy. Bioinformatic predictions were conducted to analyze the presence of interaction sites in the studied proteins. We conducted molecular modeling of proteins with unknown 3D structure: endonuclease G, CPS-6, WAH-1, and heat shock protein 70-1. These models were then refined by MolProbity server and employed together with experimentally known 3D structures of other proteins like AIF and cyclophilin A in molecular docking simulations of interactions. Fluorescence resonance energy transfer (FRET) technique and consequent image analysis was used to evaluate the interactions of fluorescently labeled proteins in cells. (en)
Title	Interactions of apoptotic proteins AIF and endonuclease G analyzed by bioinformatic predictions, molecular docking, and fluorescent microscopy Interactions of apoptotic proteins AIF and endonuclease G analyzed by bioinformatic predictions, molecular docking, and fluorescent microscopy (en)
skos:prefLabel	Interactions of apoptotic proteins AIF and endonuclease G analyzed by bioinformatic predictions, molecular docking, and fluorescent microscopy Interactions of apoptotic proteins AIF and endonuclease G analyzed by bioinformatic predictions, molecular docking, and fluorescent microscopy (en)
skos:notation	RIV/00216224:14330/08:00026398!RIV11-MSM-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06052), P(LC535), Z(MSM0021622419)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Amrichová, Jana Ulman, Vladimír Vařecha, Miroslav Kozubek, Michal Zimmermann, Michal
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	372826
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/08:00026398
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	AIF; AMID; endonuclease G; heat shock protein; cyclophilin A; CPS-6; WAH-1; colocalization; image analysis; FRET; interaction prediction; protein structure modeling; molecular docking (en)
http://linked.open.../riv/klicoveSlovo	endonuclease G FRET colocalization image analysis molecular docking AIF AMID CPS-6 WAH-1 cyclophilin A heat shock protein interaction prediction protein structure modeling
http://linked.open...ontrolniKodProRIV	[72DFFBCDC181]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Ulman, Vladimír Kozubek, Michal Vařecha, Miroslav Zimmermann, Michal Amrichová, Jana
http://linked.open...n/vavai/riv/zamer	Highly Parallel and Distributed Computing Systems
http://localhost/t...ganizacniJednotka	14330

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