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Description
| - Image cytometry applications often suffer from the lack of light due to the use of fluorescence mode. Low light levels typically cause poor signal-to-noise ratio or long exposure times. Signal level in fluorescence set-up is determined by many factors, such as power of the light source and its spectral characteristics, transmission curves of excitation/emission filters, transmission curves of and dichroic/polychroic mirrors, excitation/emission spectra of imaged fluorochrome(s), numerical aperture of the objective and its transmission curve, quantum efficiency curve of the light detector. Thus, resulting light throughput is given by the composition of spectral characteristics (functions of wavelength) of many components. Proper computation and optimization of light throughput is a non-trivial task that requires knowledge of all these curves for all available hardware components as well as all available fluorochromes.
- Image cytometry applications often suffer from the lack of light due to the use of fluorescence mode. Low light levels typically cause poor signal-to-noise ratio or long exposure times. Signal level in fluorescence set-up is determined by many factors, such as power of the light source and its spectral characteristics, transmission curves of excitation/emission filters, transmission curves of and dichroic/polychroic mirrors, excitation/emission spectra of imaged fluorochrome(s), numerical aperture of the objective and its transmission curve, quantum efficiency curve of the light detector. Thus, resulting light throughput is given by the composition of spectral characteristics (functions of wavelength) of many components. Proper computation and optimization of light throughput is a non-trivial task that requires knowledge of all these curves for all available hardware components as well as all available fluorochromes. (en)
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Title
| - Optimizing light throughput in image cytometry
- Optimizing light throughput in image cytometry (en)
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skos:prefLabel
| - Optimizing light throughput in image cytometry
- Optimizing light throughput in image cytometry (en)
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skos:notation
| - RIV/00216224:14330/07:00023729!RIV10-MSM-14330___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(LC535), Z(MSM0021622419)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14330/07:00023729
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - fluorescence microscopy; light throughput (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - Analytical Cytometry IV: Book of Abstracts
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Kozubek, Michal
- Kulaviak, Jakub
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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number of pages
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http://purl.org/ne...btex#hasPublisher
| - Česká společnost pro analytickou cytologii
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https://schema.org/isbn
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http://localhost/t...ganizacniJednotka
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is http://linked.open...avai/riv/vysledek
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