About: Fluorescent microscopy of living cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Fluorescent protein technology has started by using aequorea victoria fluorescent protein derivatives. These have become the essential tools for the cell biology research. Enhanced green fluorescent protein (EGFP) has been mutated to cyan (ECFP) and yellow (EYFP) fluorescent variants, to allow the simultaneous observation of several protein fusions in live cells especially for co-localization studies and to use the biophysical properties of these fluorophores to detect protein-protein interactions and conformational changes by fluorescence resonance energy transfer (FRET) technique. The discovery and development of red fluorescent protein variants have led to fluorophores that can be distinctly imaged together with other fluorescent proteins to achieve two- or three-color imaging. Fluorescent protein technology has started by using aequorea victoria fluorescent protein derivatives. These have become the essential tools for the cell biology research. Enhanced green fluorescent protein (EGFP) has been mutated to cyan (ECFP) and yellow (EYFP) fluorescent variants, to allow the simultaneous observation of several protein fusions in live cells especially for co-localization studies and to use the biophysical properties of these fluorophores to detect protein-protein interactions and conformational changes by fluorescence resonance energy transfer (FRET) technique. The discovery and development of red fluorescent protein variants have led to fluorophores that can be distinctly imaged together with other fluorescent proteins to achieve two- or three-color imaging. (en)
Title	Fluorescent microscopy of living cells Fluorescent microscopy of living cells (en)
skos:prefLabel	Fluorescent microscopy of living cells Fluorescent microscopy of living cells (en)
skos:notation	RIV/00216224:14330/07:00020659!RIV11-GA0-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP204/03/D031), P(LC535), Z(MSM0021622419)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Amrichová, Jana Ulman, Vladimír Vařecha, Miroslav Kozubek, Michal Zimmermann, Michal Kašík, Marek
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	422265
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/07:00020659
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorescence; microscopy; living cells; image analysis (en)
http://linked.open.../riv/klicoveSlovo	living cells fluorescence image analysis microscopy
http://linked.open...ontrolniKodProRIV	[4E4A103B6D89]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions Apoptosis-inducing factor (AIF): its translocation from mitochondria and its action on nuclear chromatin
http://linked.open...UplatneniVysledku	2007
http://linked.open...iv/tvurceVysledku	Ulman, Vladimír Kozubek, Michal Vařecha, Miroslav Zimmermann, Michal Amrichová, Jana Kašík, Marek
http://linked.open...n/vavai/riv/zamer	Highly Parallel and Distributed Computing Systems
http://localhost/t...ganizacniJednotka	14330

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