About: Automated confocal live cell microscopy based on spinning disks

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The successful development of non-invasive quantitative visualization techniques for live cell imaging have led to the development of suitable hardware and software for the acquisition and processing of multidimensional image data. Confocal spinning disk systems (based either on a classical Nipkow disk or on the microlens principle) are especially suitable for live cell imaging thanks to high acquisition speed (parallel imaging of thousands of points), high quality image detection (up to 90% quantum efficiency at negligible noise levels with state-of-the-art cameras), low photobleaching and low phototoxicity. Lately, we have been working on the optimization and automation of image acquisition and processing for this type of microscopy. This poster presents full description of our hardware set-up and several applications from live cell imaging that are carried out on this set-up in our laboratory. The successful development of non-invasive quantitative visualization techniques for live cell imaging have led to the development of suitable hardware and software for the acquisition and processing of multidimensional image data. Confocal spinning disk systems (based either on a classical Nipkow disk or on the microlens principle) are especially suitable for live cell imaging thanks to high acquisition speed (parallel imaging of thousands of points), high quality image detection (up to 90% quantum efficiency at negligible noise levels with state-of-the-art cameras), low photobleaching and low phototoxicity. Lately, we have been working on the optimization and automation of image acquisition and processing for this type of microscopy. This poster presents full description of our hardware set-up and several applications from live cell imaging that are carried out on this set-up in our laboratory. (en)
Title	Automated confocal live cell microscopy based on spinning disks Automated confocal live cell microscopy based on spinning disks (en)
skos:prefLabel	Automated confocal live cell microscopy based on spinning disks Automated confocal live cell microscopy based on spinning disks (en)
skos:notation	RIV/00216224:14330/06:00033148!RIV11-MSM-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC535), Z(MSM0021622419)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Amrichová, Jana Vařecha, Miroslav Kozubek, Michal Kozubek, Stanislav Matula, Pavel Matula, Petr Lukášová, Emilie
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	466378
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/06:00033148
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	confocal microscopy; image processing (en)
http://linked.open.../riv/klicoveSlovo	image processing confocal microscopy
http://linked.open...ontrolniKodProRIV	[19146B1A027D]
http://linked.open...in/vavai/riv/obor	JD
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions
http://linked.open...UplatneniVysledku	2006
http://linked.open...iv/tvurceVysledku	Kozubek, Stanislav Lukášová, Emilie Matula, Pavel Ondřej, Vladan Kozubek, Michal Matula, Petr Vařecha, Miroslav Amrichová, Jana
http://linked.open...n/vavai/riv/zamer	Highly Parallel and Distributed Computing Systems
http://localhost/t...ganizacniJednotka	14330

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