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rdf:type
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Description
| - We studied the cellular localization and colocalization of proteins AIF, endonuclease G and AMID, which were previously found to be involved in caspase-independent apoptosis. We designed mammalian expression vectors, which carry genes encoding the proteins of interest fused to the fluorescent proteins to study cellular localization and colocalization of these proteins. We predicted the cellular localization of the proteins of interest using software, which analyses the aminoacid sequence of the protein with various algorithms. We also designed and applied a novel method of single-cell image analysis of the translocation of the fluorescent proteins into the nucleus. Using the listed methods, we managed to confirm by software prediction the localization of AIF and endoG to mitochondria.
- We studied the cellular localization and colocalization of proteins AIF, endonuclease G and AMID, which were previously found to be involved in caspase-independent apoptosis. We designed mammalian expression vectors, which carry genes encoding the proteins of interest fused to the fluorescent proteins to study cellular localization and colocalization of these proteins. We predicted the cellular localization of the proteins of interest using software, which analyses the aminoacid sequence of the protein with various algorithms. We also designed and applied a novel method of single-cell image analysis of the translocation of the fluorescent proteins into the nucleus. Using the listed methods, we managed to confirm by software prediction the localization of AIF and endoG to mitochondria. (en)
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Title
| - Single-cell image analysis of cellular localization and colocalization of endonuclease G, AIF and AMID in living cells
- Single-cell image analysis of cellular localization and colocalization of endonuclease G, AIF and AMID in living cells (en)
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skos:prefLabel
| - Single-cell image analysis of cellular localization and colocalization of endonuclease G, AIF and AMID in living cells
- Single-cell image analysis of cellular localization and colocalization of endonuclease G, AIF and AMID in living cells (en)
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skos:notation
| - RIV/00216224:14330/06:00020052!RIV11-GA0-14330___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA202/04/0907), P(GP204/03/D031), P(GP204/05/P090), Z(MSM0021622419)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14330/06:00020052
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - living cells; image analysis; colocalization; fluorescent proteins; apoptosis (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Matula, Pavel
- Ulman, Vladimír
- Kozubek, Michal
- Matula, Petr
- Vařecha, Miroslav
- Zimmermann, Michal
- Amrichová, Jana
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http://linked.open...n/vavai/riv/zamer
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http://localhost/t...ganizacniJednotka
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