About: Evaluation of CD34+ - and Lin- -selected cells from peripheral blood stem cell grafts of patients with lymphoma during differentiation in culture ex vivo using a cDNA microarray technique

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About: Evaluation of CD34+ - and Lin- -selected cells from peripheral blood stem cell grafts of patients with lymphoma during differentiation in culture ex vivo using a cDNA microarray technique Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Objective. Hematopoietic stem cells (enriched in fraction of CD34+ cells) have the ability to regenerate hematopoiesis in all of its lineages, and this potential is clinically used in transplanting bone marrow or peripheral blood stem cells. Our objective was to assemble a suitable method for evaluating gene expression in enriched populations of hematopoietic stem cells. We compared biologic properties of cells cultured ex vivo obtained using two different ways of immunomagnetic separation (positive selection of CD34+ cells and negative selection of Lin2 cells) by means of a cDNA microarray technique. Methods. CD34+ and Lin2 cells were enriched from peripheral blood stem cell (PBSCs) grafts of patients with non-Hodgkins lymphoma. Isolated cells were in the presence of cytokine PBSCs, FLT-3 ligand, interleukin-3, interleukin-6, and granulocyte colony-stimulating factor. At days 0, 4, 6, 8, 10, 12, and 14 cells were harvested and analyzed by cDNA microarrays. Objective. Hematopoietic stem cells (enriched in fraction of CD34+ cells) have the ability to regenerate hematopoiesis in all of its lineages, and this potential is clinically used in transplanting bone marrow or peripheral blood stem cells. Our objective was to assemble a suitable method for evaluating gene expression in enriched populations of hematopoietic stem cells. We compared biologic properties of cells cultured ex vivo obtained using two different ways of immunomagnetic separation (positive selection of CD34+ cells and negative selection of Lin2 cells) by means of a cDNA microarray technique. Methods. CD34+ and Lin2 cells were enriched from peripheral blood stem cell (PBSCs) grafts of patients with non-Hodgkins lymphoma. Isolated cells were in the presence of cytokine PBSCs, FLT-3 ligand, interleukin-3, interleukin-6, and granulocyte colony-stimulating factor. At days 0, 4, 6, 8, 10, 12, and 14 cells were harvested and analyzed by cDNA microarrays. (en)
Title	Evaluation of CD34+ - and Lin- -selected cells from peripheral blood stem cell grafts of patients with lymphoma during differentiation in culture ex vivo using a cDNA microarray technique Evaluation of CD34+ - and Lin- -selected cells from peripheral blood stem cell grafts of patients with lymphoma during differentiation in culture ex vivo using a cDNA microarray technique (en)
skos:prefLabel	Evaluation of CD34+ - and Lin- -selected cells from peripheral blood stem cell grafts of patients with lymphoma during differentiation in culture ex vivo using a cDNA microarray technique Evaluation of CD34+ - and Lin- -selected cells from peripheral blood stem cell grafts of patients with lymphoma during differentiation in culture ex vivo using a cDNA microarray technique (en)
skos:notation	RIV/00216224:14330/06:00016104!RIV10-MZ0-14330___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(1A8241), P(GP301/04/P136)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Kozubek, Michal Hrabčáková, Viera Svoboda, Zbyněk Mayer, Jiří Krontorád Koutná, Irena Klabusay, Martin Krontorád, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	474686
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/06:00016104
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Hematopoietic stem cells; hematopoietic transplantation; cytokines; ex vivo expansion; immunomagnetic separation (en)
http://linked.open.../riv/klicoveSlovo	cytokines ex vivo expansion immunomagnetic separation Hematopoietic stem cells hematopoietic transplantation
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[C6173B50362B]
http://linked.open...i/riv/nazevZdroje	Experimental hematology
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	New possibilities of diagnostics of leukemia using DNA-microarrays Early diagnostics of leukemia using DNA-microchip technology and by monitoring of epigenetic changes
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	34
http://linked.open...iv/tvurceVysledku	Mayer, Jiří Svoboda, Zbyněk Kozubek, Michal Klabusay, Martin Krontorád Koutná, Irena Hrabčáková, Viera Krontorád, Petr
issn	0301-472X
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	14330
is http://linked.open...avai/riv/vysledek of	Evaluation of CD34+ - and Lin- -selected cells from peripheral blood stem cell grafts of patients with lymphoma during differentiation in culture ex vivo using a cDNA microarray technique Evaluation of CD34+ - and Lin- -selected cells from peripheral blood stem cell grafts of patients with lymphoma during differentiation in culture ex vivo using a cDNA microarray technique

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