About: Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.

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About: Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Our project is focused on study of mitochondrial apoptogenic proteins released during apoptosis and associated with caspase-independent nuclear chromatin condensation and degradation. We introduce our automated 2D/3D/4D/FRET high-resolution image data acquisition and analysis system developed to visualize and quantify fluorescence signals in fixed and living cells. For our studies, we utilize the fluorescent proteins and various low-toxicity cell permeable fluorescent probes that make it possible to conduct the non-invasive quantitative visualization in living cells. In our experiments, cells are stably or transiently transfected or cotransfected by lipofection with DNA plasmids coding fusion proteins of mitochondrial apoptogenic proteins (cytochrom c, AIF, amid, and endonuclease g) with one of fluorescence proteins (EGFP, EYFP, or t-HcRed). Our project is focused on study of mitochondrial apoptogenic proteins released during apoptosis and associated with caspase-independent nuclear chromatin condensation and degradation. We introduce our automated 2D/3D/4D/FRET high-resolution image data acquisition and analysis system developed to visualize and quantify fluorescence signals in fixed and living cells. For our studies, we utilize the fluorescent proteins and various low-toxicity cell permeable fluorescent probes that make it possible to conduct the non-invasive quantitative visualization in living cells. In our experiments, cells are stably or transiently transfected or cotransfected by lipofection with DNA plasmids coding fusion proteins of mitochondrial apoptogenic proteins (cytochrom c, AIF, amid, and endonuclease g) with one of fluorescence proteins (EGFP, EYFP, or t-HcRed). (en)
Title	Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells. Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells. (en)
skos:prefLabel	Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells. Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells. (en)
skos:notation	RIV/00216224:14330/05:00013294!RIV11-GA0-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA202/04/0907), P(GP204/03/D031), P(GP204/05/P090), Z(MSM0021622419)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Amrichová, Jana Ondřej, Vladan Ulman, Vladimír Vařecha, Miroslav Kozubek, Michal Kozubek, Stanislav Matula, Petr Lukášová, Emilie
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	524242
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/05:00013294
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	living cells; GFP; fluorescent protein; FRET; fluorescent microscopy (en)
http://linked.open.../riv/klicoveSlovo	fluorescent microscopy living cells FRET GFP fluorescent protein
http://linked.open...ontrolniKodProRIV	[E9C605BDEDEA]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	High-resolution cytomery of living cells Apoptosis-inducing factor (AIF): its translocation from mitochondria and its action on nuclear chromatin Topographic and functional characterization of telomeres in various tumor cell lines determined in situ and in vivo
http://linked.open...UplatneniVysledku	2005
http://linked.open...iv/tvurceVysledku	Kozubek, Stanislav Lukášová, Emilie Ondřej, Vladan Ulman, Vladimír Kozubek, Michal Matula, Petr Vařecha, Miroslav Amrichová, Jana
http://linked.open...n/vavai/riv/zamer	Highly Parallel and Distributed Computing Systems
http://localhost/t...ganizacniJednotka	14330

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