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Description
  • Techniques with fluorescent proteins have become very valuable tools for the study of cellular processes in living cells. These proteins enable the non-invasive quantitative visualization in vivo as molecular tags on natively non-fluorescent molecules of interest. Fluorescent proteins are welcome innovation in the field of cell biology, because in vivo experiments bring new interesting results and different point of view than in vitro and in situ experiments. This presentation will address automated 2D/3D high-resolution confocal image acquisition and analysis of cells stained with fluorescent proteins. While the approaches to automated in situ imaging of cell interior have been previously described by our group (Cytometry 45, 1-12, 2001), this contribution will concentrate on the modifications (both hardware and software ones) of our high-resolution image cytometers made for the purpose of automated in vivo imaging.
  • Techniques with fluorescent proteins have become very valuable tools for the study of cellular processes in living cells. These proteins enable the non-invasive quantitative visualization in vivo as molecular tags on natively non-fluorescent molecules of interest. Fluorescent proteins are welcome innovation in the field of cell biology, because in vivo experiments bring new interesting results and different point of view than in vitro and in situ experiments. This presentation will address automated 2D/3D high-resolution confocal image acquisition and analysis of cells stained with fluorescent proteins. While the approaches to automated in situ imaging of cell interior have been previously described by our group (Cytometry 45, 1-12, 2001), this contribution will concentrate on the modifications (both hardware and software ones) of our high-resolution image cytometers made for the purpose of automated in vivo imaging. (en)
  • Automatizované in vivo snímání buněčného obsahu s využitím fluorescenčních proteinů. (cs)
Title
  • Automatizované in vivo snímání buněčného obsahu s využitím fluorescenčních proteinů (cs)
  • Automated in vivo imaging of cell interior using fluorescent proteins
  • Automated in vivo imaging of cell interior using fluorescent proteins (en)
skos:prefLabel
  • Automatizované in vivo snímání buněčného obsahu s využitím fluorescenčních proteinů (cs)
  • Automated in vivo imaging of cell interior using fluorescent proteins
  • Automated in vivo imaging of cell interior using fluorescent proteins (en)
skos:notation
  • RIV/00216224:14330/04:00010822!RIV09-GA0-14330___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GP204/03/D031), Z(MSM 143300002)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 555652
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14330/04:00010822
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Telomere (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [394BEF7EFFB5]
http://linked.open...i/riv/nazevZdroje
  • Cytometry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 59A/2004
http://linked.open...iv/tvurceVysledku
  • Kozubek, Stanislav
  • Lukášová, Emilie
  • Ondřej, Vladan
  • Kozubek, Michal
  • Vařecha, Miroslav
  • Amrichová, Jana
http://linked.open...ain/vavai/riv/wos
  • 000221319900243
http://linked.open...n/vavai/riv/zamer
issn
  • 0196-4763
number of pages
http://localhost/t...ganizacniJednotka
  • 14330
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