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Description
| - In this study, RNase A immobilized on magnetic supports based on bead cellulose differeing in particle size and poly(HEMA-co-EDMA)microsheres were used. Enzyme was coupled to particles via cyanuric chloride method. Thermal stability of soluble and immobilzed RNase a was tested. Soluble and immobilized enzymes were preincubated in water, acetate buffer (pH 5.0) and tsic-NaCl buffer (pH 7.5) for 1 h. at different temperatures. The activity of enzyme was estimated fro UV spectrophometrical measurement. The matrices base on magnetic bead cellulose and magnetic poly(HEMA-co-EDMA) have a low tendency to adsorb RNA.
- In this study, RNase A immobilized on magnetic supports based on bead cellulose differeing in particle size and poly(HEMA-co-EDMA)microsheres were used. Enzyme was coupled to particles via cyanuric chloride method. Thermal stability of soluble and immobilzed RNase a was tested. Soluble and immobilized enzymes were preincubated in water, acetate buffer (pH 5.0) and tsic-NaCl buffer (pH 7.5) for 1 h. at different temperatures. The activity of enzyme was estimated fro UV spectrophometrical measurement. The matrices base on magnetic bead cellulose and magnetic poly(HEMA-co-EDMA) have a low tendency to adsorb RNA. (en)
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Title
| - Properties of RNase immobilized on magnetic particles
- Properties of RNase immobilized on magnetic particles (en)
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skos:prefLabel
| - Properties of RNase immobilized on magnetic particles
- Properties of RNase immobilized on magnetic particles (en)
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skos:notation
| - RIV/00216224:14310/99:00004114!RIV/2002/GA0/143102/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/99:00004114
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Properties of RNase immobilized on magnetic particles (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - 13th Bratislava International Conference on Polymers, Separation and Characterization of Macromolecules
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...ocetUcastnikuAkce
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http://linked.open...nichUcastnikuAkce
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Horák, Daniel
- Rittich, Bohuslav
- Šafář, Jan
- Španová, Alena
- Lenfeld, Jiří
- Beneš, Milan
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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number of pages
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http://purl.org/ne...btex#hasPublisher
| - Polymer Institute of the Slovak Academy of Sciences
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http://localhost/t...ganizacniJednotka
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