About: Immunomagnetic separation in the detection of Salmonella cells from foodstuffs

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cultivation method and polymerase chain reaction coupled with immunomagnetic separation (IMS-CM and IMS-PCR) are new technoques used for the detection of bacteria in foodstuffs. In this study, Salmonella was isolated from various real food samples by magnetic Dynabeads M-280 anti-Salmonella using both IMS-CM and IMS-PCR. Food samples included (milk, eggs, meat products, confectionery, vegetable, fruit products) and total 408 specimens were investigated. 49 samples (i.e. 12%) were found Salmonella positive by IMS-CM. Bacteria coupled on immunomagnetic particles were also used in IMS-PCR. Growing atypically on sensitive medium two Salmonella strains were identified using IMS-PCR. False negative results were obtained by conbventional technques. The above mentioned results demonstrated that IMS-CM and IMS-PCR techniques are more sensetive in real food samples analysis than conventional cultivation procedures. Cultivation method and polymerase chain reaction coupled with immunomagnetic separation (IMS-CM and IMS-PCR) are new technoques used for the detection of bacteria in foodstuffs. In this study, Salmonella was isolated from various real food samples by magnetic Dynabeads M-280 anti-Salmonella using both IMS-CM and IMS-PCR. Food samples included (milk, eggs, meat products, confectionery, vegetable, fruit products) and total 408 specimens were investigated. 49 samples (i.e. 12%) were found Salmonella positive by IMS-CM. Bacteria coupled on immunomagnetic particles were also used in IMS-PCR. Growing atypically on sensitive medium two Salmonella strains were identified using IMS-PCR. False negative results were obtained by conbventional technques. The above mentioned results demonstrated that IMS-CM and IMS-PCR techniques are more sensetive in real food samples analysis than conventional cultivation procedures. (en)
Title	Immunomagnetic separation in the detection of Salmonella cells from foodstuffs Immunomagnetic separation in the detection of Salmonella cells from foodstuffs (en)
skos:prefLabel	Immunomagnetic separation in the detection of Salmonella cells from foodstuffs Immunomagnetic separation in the detection of Salmonella cells from foodstuffs (en)
skos:notation	RIV/00216224:14310/99:00004086!RIV/2002/GA0/143102/N
http://linked.open.../vavai/riv/strany	68
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA203/98/1231), P(IZ3988)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Buňková, Leona Španová, Alena Rittich, Bohuslav
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	742389
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/99:00004086
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Immunomagnetic separation in the detection of Salmonella cells from foodstuffs (en)
http://linked.open.../riv/klicoveSlovo	Immunomagnetic separation in the detection of Salmonella cells from foodstuffs
http://linked.open...ontrolniKodProRIV	[832350034A83]
http://linked.open...v/mistoKonaniAkce	Compiegne
http://linked.open...i/riv/mistoVydani	Compiegne
http://linked.open...i/riv/nazevZdroje	13th Internal Symposium of Affinity Technology and Bio-Recognition
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Oriented immobilization of enzymes and antibodies using biospecific interactions Development and verification of immunological and molecular - genetical methods for the detection of selected pathogenes from the food chain.
http://linked.open...UplatneniVysledku	1999
http://linked.open...iv/tvurceVysledku	Rittich, Bohuslav Španová, Alena Karpíšková, Renata Holasová, Marie Čechová, Leona
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	1999-01-01 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Université de Technologie de Compiegne
http://localhost/t...ganizacniJednotka	14310

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