About: MALDI-TOF MS Analysis of Anaerobic Bacteria Isolated from Biofilm-Covered Surfaces in Brewery Bottling Halls     Goto   Sponge   NotDistinct   Permalink

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Description
  • Brewery filling equipment is continually exposed to moisture and nutrients during production runs and is therefore susceptible to microbial attachment and biofilm development. These biofilms play the role of reservoirs of beer spoilage microorganisms. We evaluated the usability of matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis for direct identification of beer contaminants and direct analysis of biofilm. From a total of 58 isolates obtained from the beer filling hall environment, 47% were successfully assigned to the species level and 10% to the genus level when using the commercially available BioTyper (Bruker Daltonik) database. After homemade extension of the database by eight entries belonging to genera Pectinatus, Megasphaera, and Selenomonas, the identification success rate at the species level was increased to 85%.
  • Brewery filling equipment is continually exposed to moisture and nutrients during production runs and is therefore susceptible to microbial attachment and biofilm development. These biofilms play the role of reservoirs of beer spoilage microorganisms. We evaluated the usability of matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis for direct identification of beer contaminants and direct analysis of biofilm. From a total of 58 isolates obtained from the beer filling hall environment, 47% were successfully assigned to the species level and 10% to the genus level when using the commercially available BioTyper (Bruker Daltonik) database. After homemade extension of the database by eight entries belonging to genera Pectinatus, Megasphaera, and Selenomonas, the identification success rate at the species level was increased to 85%. (en)
Title
  • MALDI-TOF MS Analysis of Anaerobic Bacteria Isolated from Biofilm-Covered Surfaces in Brewery Bottling Halls
  • MALDI-TOF MS Analysis of Anaerobic Bacteria Isolated from Biofilm-Covered Surfaces in Brewery Bottling Halls (en)
skos:prefLabel
  • MALDI-TOF MS Analysis of Anaerobic Bacteria Isolated from Biofilm-Covered Surfaces in Brewery Bottling Halls
  • MALDI-TOF MS Analysis of Anaerobic Bacteria Isolated from Biofilm-Covered Surfaces in Brewery Bottling Halls (en)
skos:notation
  • RIV/00216224:14310/14:00075472!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(EE2.3.20.0183), P(GAP503/12/1424), S
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  • 2
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  • 27013
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  • RIV/00216224:14310/14:00075472
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  • Anaerobic bacteria; Beer spoilage bacteria; Biofilm; Identification; Lactic acid bacteria; MALDI-TOF MS (en)
http://linked.open.../riv/klicoveSlovo
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  • US - Spojené státy americké
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  • [6676FC367CB0]
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  • The Journal of the American Society of Brewing Chemists
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  • 72
http://linked.open...iv/tvurceVysledku
  • Šedo, Ondrej
  • Matoulková, Dagmar
  • Vávrová, Andrea
  • Balážová, Tereza
http://linked.open...ain/vavai/riv/wos
  • 000335286900003
issn
  • 0361-0470
number of pages
http://bibframe.org/vocab/doi
  • 10.1094/ASBCJ-2014-0324-01
http://localhost/t...ganizacniJednotka
  • 14310
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